BBB Model Media Culture Media

Store at -80 degree C or 37 degree C

We deliver the 3D Human BBB model in frozen packaged with dry ice. The 3D Human BBB model can be frozen as a whole and stored at -80 degree C. 4 days prior to your experiment, you just thaw your 3D Human BBB model stored at -80 degree C.

Culture Medium
Frozen Model
Astrocytes and brain pericytes help to develop and maintain specific BBB characteristics in brain capillary endothelial cells. Co-culture of the three cell types in our 3D Human BBB model led to the enhancement of barrier properties; an increase in expressions of tight junction proteins of occludin, claudin-5 and ZO-1 and continuous localizations of ZO-1 and claudin-5. Our model mimic transport properties of the BBB due to the formation of tight junctions, higher expression of specific carriers, or great cell viability. We developed a 3D in vitro model of the BBB by culturing brain endothelial cells with pericytes and astrocytes layered in an insert. This model improves endothelial cell polarization and enhance the formation of tight junctions, provide better endothelial cell-to-cell contact that is important for barrier development, and prevent the dilution of secreted neurotrophic factors, and these conditions collectively led to the development of an in vitro model that can truly mimic the BBB.
Advantages:
1) Cells used in the 3D model are all human cells; results obtained are more relevant to human situations rather than those data from animal models, i.e. CAM et al.
2) The whole process can be monitored (from cell inoculation to the end of experiment), therefore, more crucial information can be acquired at multiple time points from a single experiment.
3) No need to perform post-experimental staining for endothelial markers, this is particularly important, if those markers are changed in experimental conditions involved in the studies. The 3D Human BBB Model contains all of the materials necessary to perform multiple angiogenesis assays in 6, 12, or 24 well formats.
The 3D Human BBB model can be use, but not limited to:
Drug BBB permeability assay
Research on BBB physiology
Cell-cell interactions
Transport pathway modulations
Research on BBB toxicology
Brain endothelial toxicity assays
Research on BBB pathology
Disease modeling
Transport and permeability studies from ions to macromolecules: effect of physiological or pathogenetic factors
Paracellular barrier and cell polarity studies: TJ protein expression, distribution, polarized distribution of transport proteins, receptors, enzymes etc
Studies on endo- and transcytosis, receptor-ligand interactions
Drug transport, drug effect on permeability, localization of receptors, polarity of drug responses
Co-culture studies: cell-cell and cell-matrix interactions
Microbial pathogenesis: virus, bacteria, parasite attachment, invasion and penetration
Compounds screening neuroimmune targets