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Leukemia Inhibitory Factor (LIF) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS555871

Recombinant human LIF contains 188 amino acids and a 16 a.a. Histidine-based tag for a total length of 204 a.a. and has a predicted molecular mass of 22.8 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of… More details

$650 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Leukemia Inhibitory Factor (LIF), Recombinant Protein
Full Product Name
ISOKine-Leukemia Inhibitory Factor (LIF), human
Product Gene Name
Host
Barley Endosperm Tissue
Species Reactivity
Human
Form/Format
Lyophilized, PBS, pH 7.2, sterile filtered
Purity/Purification
>95% by SDS-PAGE gel analysis
Preparation and Storage
Store at 4-8 degrees.
Molecular Weight
9,563 Da
3D Structure
ModBase 3D Structure for P15018
Source Note
ISOkine recombinant human LIF is produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E Coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture.
Endotoxin Level
Endotoxin Endotoxin level is less than 0.005ng per ug of ISOkine product (0.05EU/ug) as measured by turbidimetric kinetic assay.
MAT Assay
Purified ISOkine product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6 TNF-alpha and IL-1beta induction.
Applications Tested/Suitable for LIF recombinant protein
Cell Assays

Testing Data of LIF recombinant protein

Human LIF Recombinant Protein – Cat# MBS555871

Other Notes

Small volumes of LIF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LIF purchase

MBS555871 is a recombinant protein targeting LIF. Also known as ISOKine-Leukemia Inhibitory Factor (LIF), human. Reactive in Human. Host: Barley Endosperm Tissue.

Additional Details

NCBI/Uniprot data below describe general gene information for LIF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
leukemia inhibitory factor isoform 1
NCBI Official Synonym Full Names
LIF, interleukin 6 family cytokine
NCBI Official Symbol
LIF
NCBI Official Synonym Symbols
CDF; DIA; HILDA; MLPLI
UniProt Protein Name
Leukemia inhibitory factor
UniProt Synonym Protein Names
Differentiation-stimulating factor; D factor; Melanoma-derived LPL inhibitor; MLPLI; INN: Emfilermin
UniProt Gene Name
LIF
UniProt Synonym Gene Names
HILDA; LIF; D factor; MLPLI
NCBI Protein Information
leukemia inhibitory factor

NCBI Summary for LIF

The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
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UniProt Comments for LIF

LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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