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Vimentin (VIM) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS555893

Immunohistochemical staining for Vimentin is also characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin… More details

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Product Overview

Online Datasheet in PDF
Product Name
Vimentin, Polyclonal Antibody
Full Product Name
Vimentin
Product Gene Name
anti-VIM antibody
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human, Rat, Mouse, Monkey, Cow, Pig, Horse
Form/Format
Serum in liquid form plus 5mM of Sodium Azide.
Preparation and Storage
Antibody can also be aliquotted and stored frozen at -20° C in a manual defrost freezer for six months without detectable loss of activity. The antibody can be stored at 2°-8° C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
OMIM
193060
3D Structure
ModBase 3D Structure for P08670
Immunogen
Recombinant Human vimentin expressed in and purified in E Coli
Applications Tested/Suitable for anti-VIM antibody
Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-VIM antibody
Immunofluorescence: 1:2,000
Immunohistochemsitry: 1:2,000
Immunocytochemistry: 1:2,000
Western Blot: 1:5,000

Dilutions listed as a recommendation. Optimal dilution should be determined by researcher.

Immunofluorescence (IF) of anti-VIM antibody

Immunofluorescent analysis of HeLa cell culture stained with goat pAb to vimentin, MBS555893, dilution 1:500 in green, and costained with mouse mAb to actin, MBS555035 dilution 1:500 in red. Blue is Hoechst staining of nuclear DNA. MBS555893 antibody produces strong staining of intermediate filament network of the cytoskeleton, while actin antibody stains the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.
Goat Anti-VIM Polyclonal Antibody – Cat# MBS555893

Other Notes

Small volumes of anti-VIM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VIM purchase

MBS555893 is an antibody targeting VIM. Also known as Vimentin. Reactive in Human, Rat, Mouse, Monkey, Cow, Pig, Horse. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for VIM. It may not necessarily be applicable to this product.

NCBI GI #
62414289
NCBI GeneID
7431
NCBI Accession #
NP_003371.2
UniProt Primary Accession #
P08670
UniProt Related Accession #
P08670
GenBank Nucleotide #
NM_003380.5
NCBI Official Full Name
vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
UniProt Protein Name
Vimentin
Protein Family
Vimentin
UniProt Gene Name
VIM
UniProt Entry Name
VIME_HUMAN
NCBI Protein Information
vimentin

NCBI Summary for VIM

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
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UniProt Comments for VIM

Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: intermediate filament cytoskeleton; neuron projection; focal adhesion; cytoskeleton; cytoplasm; leading edge; plasma membrane; intermediate filament; peroxisome; cytosol

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens

Biological Process: Bergmann glial cell differentiation; viral reproduction; apoptosis; intermediate filament organization; cell motility; astrocyte development; cell structure disassembly during apoptosis; muscle filament sliding

Disease: Cataract 30
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Research Articles on VIM

1. shape and inner structure of these mutant filaments is significantly altered

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-VIM antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >383 publications with VIM and Nervous System Diseases
 Neoplasms Antibodies  >283 publications with VIM and Neoplasms
 Brain Diseases Antibodies  >246 publications with VIM and Brain Diseases
 Neurodegenerative Diseases Antibodies  >166 publications with VIM and Neurodegenerative Diseases
 Inflammation Antibodies  >46 publications with VIM and Inflammation
 Kidney Diseases Antibodies  >37 publications with VIM and Kidney Diseases
 Hemorrhage Antibodies  >32 publications with VIM and Hemorrhage
 Disease Models, Animal Antibodies  >32 publications with VIM and Disease Models, Animal
 Pain Antibodies  >31 publications with VIM and Pain
 Liver Diseases Antibodies  >29 publications with VIM and Liver Diseases

Organs/Tissues associated with anti-VIM antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >461 publications with VIM and Brain
 Blood Antibodies  >187 publications with VIM and Blood
 Muscle Antibodies  >101 publications with VIM and Muscle
 Nerve Antibodies  >79 publications with VIM and Nerve
 Bone Antibodies  >69 publications with VIM and Bone
 Kidney Antibodies  >52 publications with VIM and Kidney
 Liver Antibodies  >51 publications with VIM and Liver
 Bone Marrow Antibodies  >46 publications with VIM and Bone Marrow
 Eye Antibodies  >38 publications with VIM and Eye
 Vascular Antibodies  >35 publications with VIM and Vascular
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