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Aryl-Hydrocarbon Receptor Nuclear Translocator 3 (HIF-3beta/ARNTL) ELISA Kit for Donkey

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS055803

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of HIF-3B/ARNTL (hereafter termed "analyte") in undiluted original Donkey body fluids, tissue homogenates,… More details

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Aryl-Hydrocarbon Receptor Nuclear Translocator 3 (HIF-3beta/ARNTL), ELISA Kit
Full Product Name
Donkey Aryl-Hydrocarbon Receptor Nuclear Translocator 3 ELISA Kit
Product Gene Name
Species Reactivity
Donkey
Assay Type
Sandwich (Quantitative)
Detection Range
0.625ng/ml-20ng/ml.
Sensitivity
0.1ng/ml.
Specificity
No significant cross-reactivity or interference between this analyte and analogues is observed.
Preparation and Storage
Store all reagents at 2-8 degree C
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Molecular Weight
86,905 Da
3D Structure
ModBase 3D Structure for O02748

Other Notes

Small volumes of HIF-3beta/ARNTL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HIF-3beta/ARNTL purchase

MBS055803 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Aryl-Hydrocarbon Receptor Nuclear Translocator 3 (HIF-3beta/ARNTL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HIF-3beta/ARNTL. The ELISA analytical biochemical technique of the MBS055803 kit is based on HIF-3beta/ARNTL antibody-HIF-3beta/ARNTL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HIF-3beta/ARNTL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HIF-3beta/ARNTL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for HIF-3beta/ARNTL. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
aryl hydrocarbon receptor nuclear translocator
NCBI Official Symbol
ARNT
NCBI Official Synonym Symbols
AHA-1
UniProt Protein Name
Aryl hydrocarbon receptor nuclear translocator
UniProt Synonym Protein Names
Dioxin receptor, nuclear translocator; Hypoxia-inducible factor 1-beta
UniProt Gene Name
ARNT
UniProt Synonym Gene Names
ARNT protein; HIF-1-beta; HIF1-beta
UniProt Entry Name
ARNT_RABIT
NCBI Protein Information
aryl hydrocarbon receptor nuclear translocator; HIF1-beta; HIF-1 beta; HIF-1-beta; ARNT protein; hypoxia-inducible factor 1 beta; hypoxia-inducible factor 1-beta; dioxin receptor, nuclear translocator

UniProt Comments for HIF-3beta/ARNTL

Function: Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia

By similarity.

Subunit structure: Heterodimer with HIF1A

By similarity. Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. Interacts with NOCA7 and TACC3

By similarity.

Subcellular location: Nucleus.

Tissue specificity: Was expressed at almost the same level in all tissues except for the heart, liver, and small intestine.

Sequence similarities: Contains 1 bHLH (basic helix-loop-helix) domain.Contains 1 PAC (PAS-associated C-terminal) domain.Contains 2 PAS (PER-ARNT-SIM) domains.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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