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Alpha Fetoprotein (AFP) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS560255

Goats were immunized with highly purified human alpha-fetoprotein. The resulting antiserum was passed over an antigen containing immunoabsorbant from which the anti-human alpha-fetoprotein was immunoaffinity purified. More details

$350 / 1 mg +1 FREE 8GB USB
Product Documentation
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Alpha Fetoprotein (AFP), Polyclonal Antibody
Full Product Name
Affinity Purified Goat anti-Human Alpha Fetoprotein
Product Gene Name
Product Synonym Names
Affinity PurifiedGoat anti-Human Alpha Fetoprotein; anti-Human Alpha Fetoprotein
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
Unconjugated A.P.
Preparation and Storage
Store at 4 degree C.
Shelf Life: 12 months after shipping date
Molecular Weight
68,678 Da
Chromosome Location
chromosome: 4; Location: 4q11-q13
OMIM
104150
3D Structure
ModBase 3D Structure for P02771
Buffer
Phosphate buffered saline, pH 7.2
Preservative
0.1% sodium azide
Procedure
Goats were immunized with highly purified human alpha fetoprotein in Freund's adjuvant. The antiserum was solid phase absorbed to ensure it is monospecific to human alpha fetoprotein then immunoaffinity purified off an antigen containing immunoabsorbant.

Product Categories/Family for anti-AFP antibody

Application Notes for anti-AFP antibody
This product has been qualified by ELISA, immunoelectrophoresis and gel diffusion techniques.

Other Notes

Small volumes of anti-AFP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AFP purchase

MBS560255 is an antibody targeting AFP. Also known as Affinity Purified Goat anti-Human Alpha Fetoprotein. Reactive in Human. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for AFP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
alpha-fetoprotein
NCBI Official Synonym Full Names
alpha-fetoprotein
NCBI Official Symbol
AFP
NCBI Official Synonym Symbols
FETA; HPAFP; AFP
UniProt Protein Name
Alpha-fetoprotein
UniProt Synonym Protein Names
Alpha-1-fetoprotein; Alpha-fetoglobulin
Protein Family
UniProt Gene Name
AFP
UniProt Synonym Gene Names
HPAFP
UniProt Entry Name
FETA_HUMAN
NCBI Protein Information
alpha-fetoprotein; OTTHUMP00000220440; alpha-fetoglobulin; alpha-1-fetoprotein

NCBI Summary for AFP

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq]
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UniProt Comments for AFP

AFP: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Dimeric and trimeric forms have been found in addition to the monomeric form. Plasma. Synthesized by the fetal liver and yolk sac. Belongs to the ALB/AFP/VDB family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; cytoplasm

Molecular Function: metal ion binding

Biological Process: transport; progesterone metabolic process; ovulation from ovarian follicle

Disease: Alpha-fetoprotein Deficiency; Alpha-fetoprotein, Hereditary Persistence Of
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Research Articles on AFP

1. The presence of AFP mRNA in preoperative peripheral blood is related to tumor invasion of hepatocellular carcinoma.
2. The simultaneous determination of serum AFP, ferritin and IGF-1 may help in differential diagnosis of liver diseases.
3. Genetic variants in the AFP gene may be associated with HCC and cirrhosis risk for ethnic Indonesians.
4. Elevated maternal serum AFP (alpha-fetoprotein) levels were detected in second trimester in women at risk for placental insufficiency
5. Studies show that AFP enhancer can specifically enhance the expression of target TK gene within the AFP positive cell, and induce bystander effect and apoptosis in the AFP positive HepG2 cell with with GCV.
6. Multivariate analysis showed progression of AFP>15 microg/L per month and preoperative nodules>3 were associated with decreased overall survival in liver transplantation for hepatocellular carcinoma in cirrhotic patients.
7. SALL4 is a more sensitive marker than PLAP, AFP, or glypican-3 for extragonadal yolk sac tumors.
8. AFP may modulate inflammatory events in human skin.
9. Alpha-fetoprotein mRNA levels in peripheral blood of patients with hepatocellular carcinoma
10. Serum AFP level at end of interferon therapy is a possible predictor of hepatocellular carcinoma in chronic hepatitis C patients after interferon therapy.
11. All-trans-retinoic-acid resistance of hepatoma Bel 7402 cells is at least in part attributable to their high level of cytoplasmic alpha-fetoprotein.
12. Serum AFP levels ranging between 200-800 ng/ml were associated with high expression of somatostatin receptors in hepatocellular carcinoma.
13. Maternal serum alpha-fetoprotein levels are strongly related to preterm birth, but only in the context of other abnormal pregnancy markers.
14. Report novel alpha-fetoprotein-V messenger RNA isoforms in humans.
15. Serum PIVKA-II level, not serum AFP, was a valuable independent prognostic factor in HBV-related hepatocellular carcinoma.
16. Assessment of Dkk3 and AFP may be useful in the diagnosis of hepatic tumors.
17. AFP promoter enhancer increased specific expression of the human sodium iodide symporter (hNIS) for targeted radioiodine therapy of hepatocellular carcinoma.
18. Alpha-fetoprotein was more sensitive than des-gamma carboxyprothrombin and lectin-bound alpha-fetoprotein in diagnosing early hepatocellular carcinoma.
19. Case Report: Alpha-fetoprotein (AFP)-producing ovarian tumor in an elderly woman.
20. The utility of the tumour markers carcinoembryonic antigen, cancer antigen 125, carbohydrate antigen 19-9, carbohydrate antigen 15-3, alpha-fetoprotein and human chorionic gonadotropin for the diagnosis solitary pulmonary nodules, was investigated.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AFP antibody

 Reference Product  PubMed Publications
 CCR5 antibody  >2 publications with AFP and CCR5
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