• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

IgE Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS564269

MBS564269 is an antibody targeting IgE. Also known as Purified Mouse anti-Cat IgE (Clone #ME1). Host: Mouse. More details

$775 / 1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IgE, Monoclonal Antibody
Full Product Name
Purified Mouse anti-Cat IgE (Clone #ME1)
Product Gene Name
Product Synonym Names
Mouse anti-Cat IgE - Monoclonal
Clonality
Monoclonal
Host
Mouse
Form/Format
Liquid
Concentration
3.11 mg/ml (lot specific)
Preparation and Storage
Antibody should be stored between 2ºC and 8ºC for up to 14 days. For long-term storage, aliquot and store between -20ºC and -80ºC. Multiple freeze-thaw cycles are not recommended. Expiration: 12 months from shipping date.
Buffer
PBS, pH 7.2
Preservative
0.09% sodium azide
Immunogen
Cat IgE
Procedure
This mouse IgG2b kappa antibody is purified using salt fractionation procedures and Protein A chromatography. It is produced from tissue culture media obtained at our facility in The United States. Antibody concentration was determined using an absorbance at 280 nm: 1.4 equals 1.0 mg of IgG.
Quality Control Testing
Quality control tested by Indirect ELISA utilizing the following parameters.
Step Action Materials Used
1 Antigen – 500 ng/well. Incubated 60 minutes Cat IgE
2 Block plates for 30 minutes 1% BSA
3 Primary Antibody. Incubated 60 minutes Purified Mouse anti-Cat IgE (Clone #ME1)
4 HRP-conjugated Antibody. Incubated 30 minutes HRP-conjugated Goat anti-Mouse IgG2b
5 Develop with TMB. Incubated 10 minutes TMB Substrate
6 Stop reaction 0.3M H2SO4
7 Read plates at A450 Microplate Reader set at 450nm
ELISA Raw Data
Well Number Concentration A450
1 31.3 ng/mL 3.694
2 15.7 ng/mL 3.658
3 7.9 ng/mL 3.557
4 4.0 ng/mL 3.142
5 2.0 ng/mL 2.470
6 1.0 ng/mL 1.625
7 0.5 ng/mL 0.931
8 0.25 ng/mL 0.557
9 0 ng/mL 0.430
Application Notes for anti-IgE antibody
This antibody reacts with cat IgE (epsilon chain specific) as determined by ELISA and western blot. It is suitable for use in blotting assays, ELISA, and lateral Flow.

Optimal working dilutions should be determined experimentally by the investigator

ELISA Raw Data fig. of anti-IgE antibody

Mouse Anti-IgE Monoclonal Antibody – Cat# MBS564269

Other Notes

Small volumes of anti-IgE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IgE. It may not necessarily be applicable to this product.

Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000