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Glutathione S-Transferase P (GSTp) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS560900

Purified recombinant Chinese hamster ovary Glutathione S-Transferase Pi (GSTp) Protein expressed in CHO-S cells. Contains a 6xHIS tag. More details

$420 / 0.025 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Glutathione S-Transferase P (GSTp), Recombinant Protein
Full Product Name
Purified Glutathione S-Transferase P (GSTp) CHO-S
Product Gene Name
Product Synonym Names
Purified CHO GSTp Protein
Host
CHO-S Cells
Form/Format
Liquid; PBS buffer, pH 7.2, with non-protein stabilizer
Concentration
0.56 mg/ml (lot specific)
Specificity
The protein is detected by Western blotting using anti-6His and anti-GSTP antibodies. The product concentration is determined using absorbance reading at 280 nm (A0.1%280 value = 1.1) and a Bradford protein assay with a BSA standard curve.
Preparation and Storage
Store at -80°C. Following the initial thaw, please prepare multiple aliquots and store at -80°C. Multiple freeze-thaw cycles are not recommended.
OMIM
134660
3D Structure
ModBase 3D Structure for P09211
Antigen
Purified CHO GSTp Protein
Physical State
Frozen Liquid
Preservative
0.09% sodium azide
Procedure
Recombinant GSTP (GenBank: AAB39859.1) with a 6xHIS tag was expressed in and purified from CHO-S cells. Under reducing conditions, GSTP is detected at an estimated molecular weight of 25 kDa
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Quality Control Testing
Quality control tested by Direct ELISA utilizing the following parameters.

Step Action Materials Used
1 Antigen @ 25 ng/well. Incubated 60 minutes. Purified CHO GSTp Protein
2 Block plates for 30 minutes 1% BSA
3 HRP-Conjugated Antibody. Incubated 60 minutes HRP Rabbit anti-6xHIS (RHIS-45P)
4 Develop with TMB. Incubated 10 minutes TMB Substrate
5 Stop Reaction 0.3M H2SO4
6 Read plates at A450 Microplate Reader set at 450nm

Product Categories/Family for GSTp recombinant protein

Applications Tested/Suitable for GSTp recombinant protein
ELISA (EIA), Western Blot (WB)

ELISA Raw Data of GSTp recombinant protein

Well Number Concentration A450
1 62.5 ng/mL >3.8
2 31.3 ng/mL 3.150
3 15.7 ng/mL 2.084
4 7.9 ng/mL 1.054
5 4.0 ng/mL 0.638
6 2.0 ng/mL 0.282
7 1.0 ng/mL 0.153
8 0.5 ng/mL 0.091
9 0 ng/mL 0.036

QC Release Date: 06-27-2024

GSTp Recombinant Protein – Cat# MBS560900

Other Notes

Small volumes of GSTp recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GSTp purchase

MBS560900 is a recombinant protein targeting GSTp. Also known as Purified Glutathione S-Transferase P (GSTp) CHO-S. Host: CHO-S Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for GSTp. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
glutathione S-transferase P
NCBI Official Synonym Full Names
glutathione S-transferase pi 1
NCBI Official Symbol
GSTP1
NCBI Official Synonym Symbols
PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22
UniProt Protein Name
Glutathione S-transferase P
UniProt Synonym Protein Names
GST class-pi; GSTP1-1
UniProt Gene Name
GSTP1
UniProt Synonym Gene Names
FAEES3; GST3
UniProt Entry Name
GSTP1_HUMAN
NCBI Protein Information
glutathione S-transferase P

NCBI Summary for GSTp

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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