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ASFV P72 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS561097

MBS561097 is an antibody targeting ASFV P72. Also known as anti-ASFV Antibody (Mouse-Clone# 10E11)-Monoclonal. Host: Mouse. More details

$610 / 1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ASFV P72, Monoclonal Antibody
Full Product Name
anti-ASFV Antibody (Mouse-Clone# 10E11)-Monoclonal
Product Gene Name
anti-ASFV P72 antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10E11
Host
Mouse
Form/Format
Liquid. PBS, pH 7.2
Purity/Purification
Purified using Protein A chromatography
Concentration
3.36 mg/mL (lot specific)
Preparation and Storage
Aliquot and store between -20 degree C and -80 degree C. Multiple freeze-thaw cycles are not recommended.
Preservative
0.09% sodium azide
Immunogen
ASFV p72
Procedure
This mouse IgG1 kappa antibody is purified using Protein A chromatography. It is produced from tissue culture media. Antibody concentration was determined using an absorbance at 280 nm: 1.4 equals 1.0 mg of IgG.
Quality Control Testing
Quality control tested by Indirect ELISA utilizing the following parameters.
Step Action Materials Used
1 Antigen - 50 ng/well. Incubated 60 minutes ASFV p72
2 Block plates for 30 minutes 1% BSA
3 Primary Antibody. Incubated 60 minutes Purified Mouse anti-ASFV (Clone #10E11)
4 HRP-conjugated Antibody. Incubated 30 minutes HRP-conjugated Goat anti-Mouse IgG1 (GG1-90P)
5 Develop with TMB. Incubated 10 minutes TMB Substrate
6 Stop reaction 0.3M H2SO4
7 Read plates at A450 Microplate Reader set at 450nm

Product Categories/Family for anti-ASFV P72 antibody

Primary Antibody; African Swine Fever Virus (ASFV P72) Antibodies
Applications Tested/Suitable for anti-ASFV P72 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-ASFV P72 antibody
This antibody reacts with ASFV p72 protein as determined by ELISA and western blotting. Optimal working dilutions should be determined experimentally by the investigator.

ELISA Raw Data of anti-ASFV P72 antibody

Well Number Concentration A450
1 62.5 ng/mL 3.279
2 31.3 ng/mL 2.956
3 15.7 ng/mL 2.395
4 7.9 ng/mL 1.840
5 4.0 ng/mL 1.131
6 2.0 ng/mL 0.690
7 1.0 ng/mL 0.408
8 0.5 ng/mL 0.272
9 0 ng/mL 0.058
Mouse Anti-ASFV P72 Monoclonal Antibody – Cat# MBS561097

Other Notes

Small volumes of anti-ASFV P72 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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