Alpha-1 Acid Glyco (ORM1) ELISA Kit for Rat

Intended Uses: The Alpha 1 Microglobulin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Alpha 1 Microglobulin in biological fluid of Humans.

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Alpha 1 Microglobulin present in samples reacts with the anti-Alpha 1 Microglobulin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-A1M antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound A1M. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of A1M in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of A1M in the test sample. The quantity of A1M in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution!!Background/Introduction: Alpha 1 Microglobulin (A1M) is a small molecular weight glycoprotein. It is heterogeneous in charge and can occur both as a monomer or a polymer. Alpha 1 Microglobulin is produced in the liver and can be used as indicator for some renal diseases.

Small volumes of Orm1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS564100 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alpha-1 Acid Glycoprotein (Orm1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Orm1. The ELISA analytical biochemical technique of the MBS564100 kit is based on Orm1 antibody-Orm1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Orm1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Orm1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).