NSF Antibody – Mouse Monoclonal

NSF: required for intracellular membrane fusion and vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Functions as a SNAP receptor (SNARE) chaperone which binds, through soluble NSF attachment proteins (SNAPs), to SNARE complexes and utilizes the energy of ATP hydrolysis to disassemble them thus facilitating SNARE recycling. While this is a major function of NSF, it does seem to interact with other proteins, such as the AMPA receptor subunit, GluR2, and beta2-AR and is thought to affect their trafficking patterns.

Protein type: Autophagy; EC 3.6.4.6; Adaptor/scaffold; Protease; Vesicle

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: cytoplasm; cytosol; dendritic shaft; Golgi membrane; Golgi stack; lysosomal membrane; myelin sheath; plasma membrane; postsynaptic density

Molecular Function: ATP binding; ATPase activity; ATPase activity, coupled; metal ion binding; PDZ domain binding; protein binding; protein complex binding; protein kinase binding; Rab GTPase binding; syntaxin-1 binding

Biological Process: cellular protein metabolic process; COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; exocytosis; Golgi to plasma membrane protein transport; Golgi vesicle docking; intra-Golgi vesicle-mediated transport; intracellular protein transport; plasma membrane fusion; positive regulation of protein catabolic process; positive regulation of receptor recycling; post-translational protein modification; potassium ion transport; protein amino acid N-linked glycosylation via asparagine; regulation of exocytosis; synaptic transmission; vesicle-mediated transport