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Osteopontin (SPP1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS565153

Osteopontin (OPN) is an acidic extracellular matrix cell adhesion protein that is relatively abundant not only in bone matrix, plasma, urine, and milk, but is also found in malignant and atherogenic tissues. Phosphorylation, glycosylation and calcium… More details

$570 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Osteopontin (SPP1), Monoclonal Antibody
Full Product Name
Osteopontin monoclonal antibody (53)
Product Gene Name
anti-SPP1 antibody
Product Synonym Names
OPN; SPP1; Secreted phosphoprotein 1; Bone sialoprotein 1
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
53
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In PBS containing 0.1% sodium azide.
Purity/Purification
Protein G affinity purified.
Concentration
1.0 mg/ml (lot specific)
Specificity
Recognizes human Osteopontin.
Preparation and Storage
Long term storage: -20 degree C
Avoid freeze/thaw cycles.
OMIM
166490
3D Structure
ModBase 3D Structure for P10451
Immunogen
Recombinant human Osteopontin.
Source
Purified from ascites
Country of Origin
US

Product Categories/Family for anti-SPP1 antibody

Cell Organelles & Structures
Applications Tested/Suitable for anti-SPP1 antibody
ELISA (EIA), Immunohistochemistry (IHC) Formalin/Paraffin, Western Blot (WB)

Western Blot (WB) of anti-SPP1 antibody

Western blot analysis: Protein samples were resolved by SDS-PAGE on a 8-16% gradient gel, transferred to nitrocellulose, and blocked for 2 hrs in Tris-buffered saline containing 0.05% Tween 20 (TBS- T) and 5% milk powder. For immunodetection, Blots were incubated with anti-OPN 53 (5ug/ml) overnight at 4°C, followed by incubation with a HRP-conjugated goat anti-mouse secondary antibody (in TBS-T) for 1 hr at room temperature. Protein bands were visualized by enhanced chemiluminescence on Kodak X-OMAT film. M= Magic Mark.
Mouse Anti-SPP1 Monoclonal Antibody – Cat# MBS565153

SDS-PAGE of anti-SPP1 antibody

SDS-PAGE analysis of MBS565153: Lane 1: MW Marker, Lane 2 and Lane 3: 2ug of Osteopontin monoclonal antibody (53), resolved under reduced and non-reduced condition.
Mouse Anti-SPP1 Monoclonal Antibody, SDS-PAGE – Cat# MBS565153

Immunohistochemistry (IHC) of anti-SPP1 antibody

Immunohistochemistry analysis of formalin-fixed, paraffin-embedded breast cancer tissue stained with 2ug/ml anti-OPN 53.
Mouse Anti-SPP1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS565153

Other Notes

Small volumes of anti-SPP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPP1 purchase

MBS565153 is an antibody targeting SPP1. Also known as Osteopontin monoclonal antibody (53). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SPP1. It may not necessarily be applicable to this product.

NCBI GI #
129260
NCBI GeneID
6696
NCBI Accession #
P10451.1
UniProt Primary Accession #
P10451
UniProt Secondary Accession #
Q15681; Q15682; Q15683; Q4W597; Q567T5; Q8NBK2; Q96IZ1; B2RDA1
UniProt Related Accession #
P10451
NCBI Official Full Name
Osteopontin
NCBI Official Synonym Full Names
secreted phosphoprotein 1
NCBI Official Symbol
SPP1
NCBI Official Synonym Symbols
OPN; BNSP; BSPI; ETA-1
UniProt Protein Name
Osteopontin
UniProt Synonym Protein Names
Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin
Protein Family
COMPASS component
UniProt Gene Name
SPP1
UniProt Synonym Gene Names
BNSP; OPN; SPP-1
UniProt Entry Name
OSTP_HUMAN
NCBI Protein Information
osteopontin

NCBI Summary for SPP1

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
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UniProt Comments for SPP1

osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: apical part of cell; cell projection; extracellular region; extracellular space; perinuclear region of cytoplasm

Molecular Function: cytokine activity; extracellular matrix binding; protein binding

Biological Process: biomineral formation; cell adhesion; decidualization; embryo implantation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; inflammatory response; negative regulation of collateral sprouting of intact axon in response to injury; neutrophil chemotaxis; osteoblast differentiation; positive regulation of bone resorption; response to steroid hormone stimulus; response to vitamin D
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Research Articles on SPP1

1. SPP1 SNPs at -443 C>T and -1748 G>A loci may be useful markers for predicting the response to PEG-IFN-alpha-2b plus RBV therapy in Egyptian patients with chronic HCV genotype 4 infection.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SPP1 antibody

 Reference Product  PubMed Publications
 RUNX2 antibody  >143 publications with SPP1 and RUNX2
 CD44 antibody  >130 publications with SPP1 and CD44
 BMP2 antibody  >77 publications with SPP1 and BMP2
 THBS1 antibody  >14 publications with SPP1 and THBS1
 MMP7 antibody  >11 publications with SPP1 and MMP7
 ACP5 antibody  >7 publications with SPP1 and ACP5
 TP53 antibody  >4 publications with SPP1 and TP53
 ITGB1 antibody  >2 publications with SPP1 and ITGB1
 ITGAV antibody  >2 publications with SPP1 and ITGAV

Pathways associated with anti-SPP1 antibody

 Products by Pathway  Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 FGF Signaling Pathway antibodies  FGF Signaling Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram

Diseases associated with anti-SPP1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >262 publications with SPP1 and Inflammation
 Kidney Diseases Antibodies  >233 publications with SPP1 and Kidney Diseases
 Fibrosis Antibodies  >165 publications with SPP1 and Fibrosis
 Neoplasm Metastasis Antibodies  >139 publications with SPP1 and Neoplasm Metastasis
 Necrosis Antibodies  >132 publications with SPP1 and Necrosis
 Calcinosis Antibodies  >116 publications with SPP1 and Calcinosis
 Adenocarcinoma Antibodies  >111 publications with SPP1 and Adenocarcinoma
 Heart Diseases Antibodies  >108 publications with SPP1 and Heart Diseases
 Neoplasms, Experimental Antibodies  >85 publications with SPP1 and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >79 publications with SPP1 and Breast Neoplasms

Organs/Tissues associated with anti-SPP1 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >1106 publications with SPP1 and Bone
 Blood Antibodies  >562 publications with SPP1 and Blood
 Kidney Antibodies  >369 publications with SPP1 and Kidney
 Vascular Antibodies  >356 publications with SPP1 and Vascular
 Connective Tissue Antibodies  >343 publications with SPP1 and Connective Tissue
 Muscle Antibodies  >291 publications with SPP1 and Muscle
 Bone Marrow Antibodies  >198 publications with SPP1 and Bone Marrow
 Mouth Antibodies  >186 publications with SPP1 and Mouth
 Liver Antibodies  >137 publications with SPP1 and Liver
 Heart Antibodies  >137 publications with SPP1 and Heart
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