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HSP70 cofactor; HSP24 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS565222

GrpE (Glucose Regulated Protein E Coli) is encoded by a monocistronic E Coli gene, which is under the control of both s32 and s70 transcription factors. The GrpE protein is a so-called co-chaperone because it is known to assist the DnaK (Hsp70) protein to… More details

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Product Overview

Online Datasheet in PDF
Product Name
GrpE (HSP70 cofactor; HSP24), Polyclonal Antibody
Full Product Name
GrpE (E Coli) polyclonal antibody
Product Synonym Names
GrpE
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
E Coli
Form/Format
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Purity/Purification
Protein A affinity purified.
Specificity
Recognizes bacteria GrpE. Detects a band of ~24kDa by Western blot.
Preparation and Storage
Long term storage: -20 degree C
Avoid freeze/thaw cycles.
Molecular Weight
21,798 Da
3D Structure
ModBase 3D Structure for P09372
Immunogen
Purified E Coli GrpE.
Applications Tested/Suitable for anti-HSP70 cofactor; HSP24 antibody
Western Blot (WB)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~24kDa by Western blot.

Western Blot (WB) of anti-HSP70 cofactor; HSP24 antibody

Western blot analysis of GrpE: Lane 1: MW marker, Lane 2: GrpE Recombinant E Coli Protein.
Rabbit Anti-HSP70 cofactor; HSP24 Polyclonal Antibody – Cat# MBS565222

Other Notes

Small volumes of anti-HSP70 cofactor; HSP24 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HSP70 cofactor; HSP24 purchase

MBS565222 is an antibody targeting HSP70 cofactor; HSP24. Also known as GrpE (E Coli) polyclonal antibody. Reactive in E Coli. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HSP70 cofactor; HSP24. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
NCBI Official Full Name
Protein GrpE
NCBI Official Symbol
grpE
NCBI Official Synonym Symbols
ECK2610; JW2594
UniProt Protein Name
Protein GrpE
UniProt Synonym Protein Names
HSP-70 cofactor; HSP24; Heat shock protein B25.3
Protein Family
UniProt Gene Name
grpE
UniProt Entry Name
GRPE_ECOLI
NCBI Protein Information
heat shock protein

NCBI Summary for HSP70 cofactor; HSP24

HT_Cmplx22_Cyt: [More information is available at EcoGene: EG10416]. Review: [More information is available at EcoCyc: EG10416].

UniProt Comments for HSP70 cofactor; HSP24

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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