Rabbit Anti-[K(ac)40] Alpha -Tubulin Polyclonal Antibody – Cat# MBS565266

Treatment of A549 cells with the histone deacetylase inhibitor trichostatin A (TSA, 0.3 µM, 7 hr.) induces acetylation of alpha-tubulin-Lys40. Western blot analysis was performed with A549 cell extracts (100,000 cells per lane) from either vehicle treated (Control) or TSAtreated (+TSA) cells. All lanes were probed with a 1:1000 dilution of the primary antibody, anti-alpha-tubulin (K(Ac)40) . For lanes 2 and 5 the primary antibody was preincubated with the immunogen peptide (Ac-QMPSD-K(Ac)-TIGGG[CNH2] and for lanes 3 and 6 it was preincubated with non-acetylated peptide Ac-QMPSDKTIGGG[C-NH2] (peptides: 0.5 µg/µl, antibody: 1:100 dilution, 37 degree C, 1hr.). Secondary antibody for all lanes was donkey anti-rabbit-AP (1:2000; Jackson Immunoresearch) and the nitrocellulose membrane was developed with BCIP/NBT.

[K(ac)40] Alpha -Tubulin Antibody – Rabbit Polyclonal

Name: [K(ac)40] Alpha -Tubulin Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS565266
Price: 725 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS565266

MBS565266 is an antibody targeting [K(Ac)40] alpha -Tubulin. Also known as [K(Ac)40] alpha -Tubulin polyclonal antibody. Reactive in Chicken, Primate, Rodent, Xenopus, Zebrafish. Host: Rabbit. More details

$725 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

[K(Ac)40] alpha -Tubulin, Polyclonal Antibody

Full Product Name

[K(Ac)40] alpha -Tubulin polyclonal antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Chicken, Primate, Rodent, Xenopus, Zebrafish

Form/Format

Liquid. In PBS.

Specificity

Recognizes primate, rodent, chicken, Xenopus and zebrafish alpha-tubulin. Detects a band of ~50kDa by Western blot.

Preparation and Storage

Long term: -20 degree C
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20 degree C.

Immunogen

Synthetic acetylated peptide (K(Ac)40) corresponding to aa 35-45 (Q35MPSD-K(Ac)-TIGGG45) of human alpha -tubulin.

Product Categories/Family for anti-[K(Ac)40] alpha -Tubulin antibody

Cell Organelles & Structures; Histone Posttranslational Modifications; Stem Cells

Applications Tested/Suitable for anti-[K(Ac)40] alpha -Tubulin antibody

Western Blot (WB)

Application Notes for anti-[K(Ac)40] alpha -Tubulin antibody

Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~50kDa by Western blot.

Other Notes

Small volumes of anti-[K(Ac)40] alpha -Tubulin antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for [K(Ac)40] alpha -Tubulin. It may not necessarily be applicable to this product.

Protein Family

Alpha-tubulin N-acetyltransferase

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.