Rabbit Anti-HMOX2 Polyclonal Antibody – Cat# MBS565778

Western blot analysis: HO-1 was detected using HO-1, pAb (lanes 1, 3, 5 and 7), and HO-2 was detected using HO-2, pAb (lanes 2, 4, 6 and 8). Both antibodies were used at a dilution of 1:1000. Luminograph (ECL, 15 sec. exposure, ref. FM020/054) showing HO-1 and HO-2 expression in lysates prepared from gIFN/LPS stimulated RAW264.7 mouse macrophage cells (lanes 1 and 2), HepG2 (lanes 3 and 4), rat testis (HP9312, lanes 5 and 6) and rat spleen (HP9313, lanes 7 and 8).

Heme Oxygenase 2 (HMOX2) Antibody – Rabbit Polyclonal

Name: HMOX2 Antibody; Polyclonal; Rabbit; Human/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS565778
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS565778

Fixatives: Several fixative solutions may be used. Aldehyde-combination fixatives (i.e. those containing formaldehyde and glutaraldehyde) usually give satisfactory results. Bouin and Susa fixatives containing 0.1-0.2% glutaraldehyde have been used… More details

$675 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Heme oxygenase 2 (HMOX2), Polyclonal Antibody

Full Product Name

Heme oxygenase 2 polyclonal antibody

Product Gene Name

anti-HMOX2 antibody

Product Synonym Names

HMOX2; Hsp32; Heat shock protein 32; HO-2; Heme oxygenase 2, pAb

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Rat

Purity/Purification

Protein G-affinity purified.

Specificity

Recognizes human and rat heme oxygenase 2.

Preparation and Storage

Long Term: -20 degree C
Avoid freeze/thaw cycles.

Molecular Weight

32,837 Da

OMIM

D21243 mRNA

3D Structure

ModBase 3D Structure for P30519

Immunogen

Synthetic peptide corresponding to aa 246-264 of human heme oxygenase 2 (HO-2) protein (E.C. 1.14.99.3).

Note

The antiserum was raised to a synthetic peptide, sequence: H-E-T-L-E-D-G-F-P-V-H-D-G-K-G-D-M-R-K-C-NH₂ corresponding to amino acid residues 246-264, in amidated form, of the human HO-2 protein (McCoubrey et al., 1992). Two amino acid substitutions (Phe252 --> Leu; Met261 --> Val) exist in the aligned region within rat HO-2, and four (Leu248 --> Val; Gly251 --> Arg; Phe252 --> Leu; Met261 --> Val) in rabbit HO-2. The peptide was conjugated at the C-terminal cysteine residue via a maleimido linkage to keyhole limpet haemocyanin. The antiserum has been purified by caprylic acid and ammonium sulphate precipitation, and by protein G affinity chromatography.

Application: Heme oxygenase-2 is constitutively expressed in many tissues, but the protein levels are extremely variable. The use of enriched enzyme preparations is recommended for experimental analysis. This antibody has been shown to react strongly with HO-2 on Western blots of microsomal preparations of unstimulated rat brain (HP 9311), testis (HP 9312) and to a lesser extent with liver (HP 9314). Strong bands have also been detected in extracts of whole rat brain and HepG2, a human hepatocellular carcinoma cell line (# HB8065). A single band at ~36kDa is observed at a primary antibody dilution of 1:2000 or above when detected using enhanced chemiluminescence. Antibody HC 3002 is believed to be wholly specific for HO-2 showing no cross-reactivity with HO-1 in all systems studied to date. The immunostaining is fully abolished by pre-adsorption of the antibody with cognate peptide (HP 9302). The antibody has been used successfully in immunocytochemical studies of HO-2 localisation in human bronchial epithelium biopsy material.

Fixatives: Several fixative solutions may be used. Aldehyde-combination fixatives (i.e. those containing formaldehyde and glutaraldehyde) usually give satisfactory results. Bouin and Susa fixatives containing 0.1-0.2% glutaraldehyde have been used satisfactorily.

Product Categories/Family for anti-HMOX2 antibody

Protein Synthesis & Folding; Oxidative Stress

Applications Tested/Suitable for anti-HMOX2 antibody

Immunohistochemistry (IHC), Western Blot (WB)

Other Notes

Small volumes of anti-HMOX2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HMOX2 purchase

MBS565778 is an antibody targeting HMOX2. Also known as Heme oxygenase 2 polyclonal antibody. Reactive in Human, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HMOX2. It may not necessarily be applicable to this product.

NCBI GI #

1170328

NCBI GeneID

3163

NCBI Accession #

P30519.2

UniProt Primary Accession #

P30519

UniProt Secondary Accession #

O60605; A8MT35; D3DUD5; I3L430

UniProt Related Accession #

P30519

NCBI Official Full Name

Heme oxygenase 2

NCBI Official Synonym Full Names

heme oxygenase 2

NCBI Official Symbol

HMOX2

NCBI Official Synonym Symbols

HO-2

UniProt Protein Name

Heme oxygenase 2

Protein Family

Heme oxygenase

UniProt Gene Name

HMOX2

UniProt Synonym Gene Names

HO2; HO-2

NCBI Protein Information

heme oxygenase 2

NCBI Summary for HMOX2

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Several alternatively spliced transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

UniProt Comments for HMOX2

HMOX2: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter. Belongs to the heme oxygenase family.

Protein type: Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.14.99.3; Endoplasmic reticulum; Oxidoreductase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: endoplasmic reticulum membrane; membrane; plasma membrane

Molecular Function: heme binding; heme oxygenase (decyclizing) activity; protein binding

Biological Process: cellular iron ion homeostasis; heme catabolic process; heme oxidation; iron ion homeostasis; response to hypoxia; response to oxidative stress

Research Articles on HMOX2

1. The present study suggests a weak association between HMOX2 rs1051308 polymorphism and the risk to develop multiple sclerosis (MS) in Spanish Caucasian men and a trend towards association between the HMOX1 rs2071746A and MS risk.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HMOX2 antibody

Reference Product	PubMed Publications
HMOX1 antibody	>18 publications with HMOX2 and HMOX1
JUN antibody	>1 publications with HMOX2 and JUN
FOS antibody	>1 publications with HMOX2 and FOS
NFE2L2 antibody	>1 publications with HMOX2 and NFE2L2
BLVRA antibody	>1 publications with HMOX2 and BLVRA

Pathways associated with anti-HMOX2 antibody

Products by Pathway	Pathway Diagram
Heme Degradation Pathway antibodies	Heme Degradation Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
Iron Uptake And Transport Pathway antibodies	Iron Uptake And Transport Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Porphyrins Pathway antibodies	Metabolism Of Porphyrins Pathway Diagram
Mineral Absorption Pathway antibodies	Mineral Absorption Pathway Diagram
Mineral Absorption Pathway antibodies	Mineral Absorption Pathway Diagram
Neutrophil Degranulation Pathway antibodies	Neutrophil Degranulation Pathway Diagram
Porphyrin And Chlorophyll Metabolism Pathway antibodies	Porphyrin And Chlorophyll Metabolism Pathway Diagram

Diseases associated with anti-HMOX2 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>5 publications with HMOX2 and Nervous System Diseases
Inflammation Antibodies	>3 publications with HMOX2 and Inflammation
Cardiovascular Diseases Antibodies	>2 publications with HMOX2 and Cardiovascular Diseases
Necrosis Antibodies	>2 publications with HMOX2 and Necrosis
Immune System Diseases Antibodies	>1 publications with HMOX2 and Immune System Diseases
Pain Antibodies	>1 publications with HMOX2 and Pain
Fatty Liver Antibodies	>1 publications with HMOX2 and Fatty Liver
Liver Diseases Antibodies	>1 publications with HMOX2 and Liver Diseases
Hypertension Antibodies	>1 publications with HMOX2 and Hypertension
Hyperplasia Antibodies	>1 publications with HMOX2 and Hyperplasia

Organs/Tissues associated with anti-HMOX2 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>5 publications with HMOX2 and Liver
Blood Antibodies	>4 publications with HMOX2 and Blood
Brain Antibodies	>4 publications with HMOX2 and Brain
Muscle Antibodies	>2 publications with HMOX2 and Muscle
Vascular Antibodies	>1 publications with HMOX2 and Vascular
Ear Antibodies	>1 publications with HMOX2 and Ear
Heart Antibodies	>1 publications with HMOX2 and Heart
Eye Antibodies	>1 publications with HMOX2 and Eye
Skin Antibodies	>1 publications with HMOX2 and Skin
Testis Antibodies	>1 publications with HMOX2 and Testis