Rabbit Anti-USP18 Polyclonal Antibody – Cat# MBS565792

Figure: Detection of purified recombinant USP18 (0.5ug) by Western blot using USP18 (human), pAb (1:1'000).

USP18 Antibody – Rabbit Polyclonal

Name: USP18 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS565792
Price: 490 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS565792

USP18 (UBP43) is a specific deconjugating enzyme for ISG15, a vertebrate ubiquitin like protein strongly upregulated by type I interferon following bacterial or viral infection. Whilst the molecular and regulatory consequences of ISGylation are not fully… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

USP18, Polyclonal Antibody

Full Product Name

USP18 (human) polyclonal antibody

Product Gene Name

anti-USP18 antibody

Product Synonym Names

UBP43; ISG15 specific protease; USP18 (human), pAb

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid. In PBS containing 0.01% sodium azide.For further dilutions use PBS pH 7.4 and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).

Purity/Purification

Purified by salt precipitation.

Specificity

Recognizes human USP18.

Preparation and Storage

Long Term: -20 degree C
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20 degree C.
Stable for at least 1 year after receipt when stored as recommended.

Molecular Weight

41,352 Da

OMIM

AF176642 mRNA

3D Structure

ModBase 3D Structure for Q9UMW8

Immunogen

Synthetic peptide corresponding to aa 89-100 of human USP18.

Product Categories/Family for anti-USP18 antibody

Ubiquitin & UBL Signaling; Posttranslational Modifications; Protein Synthesis & Folding; Proteolysis & Protein Degradation; Stem Cells

Applications Tested/Suitable for anti-USP18 antibody

Western Blot (WB)

Application Notes for anti-USP18 antibody

Western Blot (1:1'000).

Other Notes

Small volumes of anti-USP18 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for USP18 purchase

MBS565792 is an antibody targeting USP18. Also known as USP18 (human) polyclonal antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for USP18. It may not necessarily be applicable to this product.

NCBI GI #

10720335

NCBI GeneID

11274

NCBI Accession #

Q9UMW8.1

UniProt Primary Accession #

Q9UMW8

UniProt Secondary Accession #

Q53Y90; Q6IAD9; Q9NY71

UniProt Related Accession #

Q9UMW8

NCBI Official Full Name

Ubl carboxyl-terminal hydrolase 18

NCBI Official Synonym Full Names

ubiquitin specific peptidase 18

NCBI Official Symbol

USP18

NCBI Official Synonym Symbols

ISG43; UBP43

UniProt Protein Name

Ubl carboxyl-terminal hydrolase 18

UniProt Synonym Protein Names

43 kDa ISG15-specific protease; hUBP43; ISG15-specific-processing protease; Ubl thioesterase 18

Protein Family

Ubl carboxyl-terminal hydrolase

UniProt Gene Name

USP18

UniProt Synonym Gene Names

ISG43; hUBP43

NCBI Protein Information

ubl carboxyl-terminal hydrolase 18

NCBI Summary for USP18

The protein encoded by this gene belongs to the ubiquitin-specific proteases (UBP) family of enzymes that cleave ubiquitin from ubiquitinated protein substrates. It is highly expressed in liver and thymus, and is localized to the nucleus. This protein efficiently cleaves only ISG15 (a ubiquitin-like protein) fusions, and deletion of this gene in mice results in a massive increase of ISG15 conjugates in tissues, indicating that this protein is a major ISG15-specific protease. Mice lacking this gene are also hypersensitive to interferon, suggesting a function of this protein in downregulating interferon responses, independent of its isopeptidase activity towards ISG15. [provided by RefSeq, Sep 2011]

UniProt Comments for USP18

USP18: Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms. Belongs to the peptidase C19 family.

Protein type: EC 3.1.2.-; EC 3.4.19.-; Protease; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: cytosol; nucleus

Molecular Function: ISG15-specific protease activity; protein binding; ubiquitin-specific protease activity

Research Articles on USP18

1. USP18 negatively regulates NF-kappaB signaling by targeting TAK1 and NEMO for deubiquitination through distinct mechanisms.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-USP18 antibody

Reference Product	PubMed Publications
ISG15 antibody	>79 publications with USP18 and ISG15
STAT1 antibody	>34 publications with USP18 and STAT1
IFIT1 antibody	>11 publications with USP18 and IFIT1
JAK1 antibody	>9 publications with USP18 and JAK1
TAB1 antibody	>2 publications with USP18 and TAB1
UBE2E1 antibody	>1 publications with USP18 and UBE2E1

Pathways associated with anti-USP18 antibody

Products by Pathway	Pathway Diagram
Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies	Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Deubiquitination Pathway antibodies	Deubiquitination Pathway Diagram
ISG15 Antiviral Mechanism Pathway antibodies	ISG15 Antiviral Mechanism Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Interferon Signaling Pathway antibodies	Interferon Signaling Pathway Diagram
Interferon Alpha/beta Signaling Pathway antibodies	Interferon Alpha/beta Signaling Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Post-translational Protein Modification Pathway antibodies	Post-translational Protein Modification Pathway Diagram
Regulation Of IFNA Signaling Pathway antibodies	Regulation Of IFNA Signaling Pathway Diagram

Diseases associated with anti-USP18 antibody

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>22 publications with USP18 and Liver Diseases
Inflammation Antibodies	>14 publications with USP18 and Inflammation
Nervous System Diseases Antibodies	>12 publications with USP18 and Nervous System Diseases
Disease Models, Animal Antibodies	>6 publications with USP18 and Disease Models, Animal
Necrosis Antibodies	>6 publications with USP18 and Necrosis
Carcinoma, Hepatocellular Antibodies	>4 publications with USP18 and Carcinoma, Hepatocellular
Lung Diseases Antibodies	>3 publications with USP18 and Lung Diseases
Fibrosis Antibodies	>3 publications with USP18 and Fibrosis
Liver Neoplasms Antibodies	>2 publications with USP18 and Liver Neoplasms
Neoplasms, Experimental Antibodies	>2 publications with USP18 and Neoplasms, Experimental

Organs/Tissues associated with anti-USP18 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>34 publications with USP18 and Blood
Liver Antibodies	>29 publications with USP18 and Liver
Brain Antibodies	>12 publications with USP18 and Brain
Lung Antibodies	>11 publications with USP18 and Lung
Bone Antibodies	>8 publications with USP18 and Bone
Vascular Antibodies	>7 publications with USP18 and Vascular
Muscle Antibodies	>6 publications with USP18 and Muscle
Uterus Antibodies	>5 publications with USP18 and Uterus
Intestine Antibodies	>4 publications with USP18 and Intestine
Kidney Antibodies	>2 publications with USP18 and Kidney