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Irisin (FNDC5) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS565860

Irisin is a hormone that is involved in converting white adipose tissue into brown adipose tissue after physical exercise. It is formed after the membrane bound protein fibronectin type III domain containing protein 5 (FNDC5) is cleaved resulting in a… More details

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Product Overview

Online Datasheet in PDF
Product Name
Irisin (FNDC5), Active Protein
Full Product Name
Irisin (human), (recombinant) (active)
Product Gene Name
Host
CHO cells
Form/Format
Liquid. In 1X PBS
Purity/Purification
>=90% (SDS-PAGE)
Preparation and Storage
Stable for up to a week when stored at 4 degree C and at least 6 months when stored at -80 degree C. Avoid freeze/thaw cycles
Shipping: Shipped on Dry Ice
Molecular Weight
15,527 Da
OMIM
611906
3D Structure
ModBase 3D Structure for Q8NAU1
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Source Note
aa 32-143, also known as Irisin, comprises the majority of the extracellular domain of FNDC5. It is fused at the N-terminus to a DYKDDDDK-tag with an 8 aa linker between the DYKDDDDK-tag and Irisin
Endotoxin
<0.01EU/ug
Biological Activity
Treatment of 3T3L1 with 2ug/ml Irisin induces UCP-1 expression

Product Categories/Family for FNDC5 active protein

Applications Tested/Suitable for FNDC5 active protein
ELISA (EIA), Western Blot (WB), SDS-PAGE, Activity assay

Other Notes

Small volumes of FNDC5 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FNDC5 purchase

MBS565860 is an active protein targeting FNDC5. Also known as Irisin (human), (recombinant) (active). Host: CHO cells.

Additional Details

NCBI/Uniprot data below describe general gene information for FNDC5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
fibronectin type III domain-containing protein 5 isoform 3 preproprotein
NCBI Official Synonym Full Names
fibronectin type III domain containing 5
NCBI Official Symbol
FNDC5
NCBI Official Synonym Symbols
FRCP2; irisin
UniProt Protein Name
Fibronectin type III domain-containing protein 5
UniProt Synonym Protein Names
Fibronectin type III repeat-containing protein 2Irisin
UniProt Gene Name
FNDC5
UniProt Synonym Gene Names
FRCP2
UniProt Entry Name
FNDC5_HUMAN
NCBI Protein Information
fibronectin type III domain-containing protein 5; fibronectin type III repeat-containing protein 2

NCBI Summary for FNDC5

This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
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UniProt Comments for FNDC5

FNDC5: Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: peroxisomal membrane; endoplasmic reticulum; plasma membrane; integral to membrane; extracellular region

Molecular Function: hormone activity

Biological Process: response to muscle activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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