DnaJ Recombinant Protein

Escherichia coli heat shock protein DnaJ belongs to the molecular chaperone class of proteins. Located in an operon with dnaK, the E Coli dnaJ gene shares sequence identity with eukaryotic cytosolic and endoplasmic reticulum DnaJ homologs (Hsp40) involved in protein folding, membrane translocation of newly synthesized proteins, and initiation of translation. The N-terminal, highly-conserved "J" domain of the DnaJ protein supports interaction with the DnaK protein and represents the signature sequence of DnaJ family members. Bacterial DnaJ protein acts synergistically with bacterial chaperones DnaK (Hsp70 homolog) and GrpE in various functions, including suppressing eukaryotic and prokaryotic polypeptide aggregation to promote protein folding, facilitating protein translocation through intracellular compartments or protein secretion, and repairing and reactivating partially aggregated enzymes such as E Coli RNAP and luciferase. In these reactions, DnaJ protein facilitates the binding of DnaK to its substrate by accelerating DnaK-catalyzed ATP hydrolysis to produce a DnaK-ADP form with a higher affinity for certain protein substrates. Some eukaryotic homologues of DnaJ also display an ability to stimulate the ATPase activity of Hsp70 proteins, including DnaJ homologue S. cerevisiae YDJ1 which interacts with DnaK homologue Ssal to stimulate its ATPase activity. Data suggests that DnaJ protein acting alone possesses chaperone activity, demonstrating an ability to bind tightly to denatured proteins such as rhodanese or luciferase to prevent their aggregation or misfolding.

Small volumes of DnaJ recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS565953 is a recombinant protein targeting DnaJ. Also known as DnaJ (E Coli), (recombinant) (His-tag). Host: E Coli.