Netrin-1 (NTN1) Human Recombinant Protein

HEK293 cells

Human, Mouse, Rat

Lyophilized. Contains PBS

>=90% (SDS-PAGE)
Purified by multi-step chromatography

0.1mg/ml after reconstitution (lot specific)

Tests positive for binding to human Unc5b by ELISA. Previous lots produced from the same cell line bind to human, mouse and rat receptors UNC5H and DCC

Ships on Blue Ice
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C.
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

~85 kDa

Chromosome: 17; NC_000017.10 (8924859..9147317). Location: 17p13-p12

601614

ModBase 3D Structure for O95631

The extracellular domain of human netrin-1 (aa 28-604) is fused at the N-terminus to a DYKDDDDK®-tag.

Reconstitute with 100µl sterile distilled water. Resuspended protein should be used immediately upon reconstitution to avoid loss in activity due to non-specific binding to the vial. Further dilutions should be made with medium containing 5% fetal calf serum or 0.5% BSA.

The Netrins (whose name means “one who guides” in Sanskrit) are a family of laminin-related secreted protein. In mice, human and rat, netrin-1, netrin-3/NTL2, Netrin-4 and G-Netrins have been cloned. Netrin-1 is predominantly expressed by floor plate cells as commissural axons extend toward the ventral midline. Netrin-1 induces axonal outgrowth, axon orientation and neuronal migration in neuronal development. It is chemoattractive for some neurons and chemorepellent for others. To carry its functions, netrin-1 interacts with specific receptors: DCC/Neogenin and UNC-5. These receptors belong to the family of “dependence receptors” that induce apoptosis in the absence of their respective ligand, and promote survival when bound to their ligand. The Netrin-1 mediated survival signal inhibits p53-induced apoptosis. Netrin-1 is also expressed in organs such as pancreas, lung, bowel, bone and mammary gland. In these tissues, netrin-1 has different roles such as invasion of epithelial cells, leukocytes migration, cell adhesion or angiogenesis. Netrin-1, as a survival factor, also contributes to the control of tumorigenesis. Historical results have shown that netrin-1 induces axon outgrowth and inhibits DCC- and UNC5H2-mediated apoptosis in HEK 293 cells.

Cellular Analysis; Cell Signaling