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Tnc:tweak (TNC) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS566260

Some TNF ligands (such as TWEAK (mouse) or FasL (mouse)) produced as soluble forms do not assemble into stable trimers and show low receptor binding. Addition of the tenascin-C oligomerization domain (TNC) (from chicken) at the N-terminus of recombinant mouse… More details

Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TNC:TWEAK, Recombinant Protein
Full Product Name
TNC:TWEAK, (soluble) (mouse), (recombinant)
Product Gene Name
Host
HEK293 cells
Form/Format
Lyophilized. Contains PBS
Purity/Purification
>=95% (SDS-PAGE)
Concentration
0.1mg/ml after reconstitution (lot specific)
Specificity
Binds to Fn14 (mouse):Fc (human) (rec.)
Preparation and Storage
Stable for at least 6 months after receipt when stored at -20 degree C
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20 degree C
Shipping: Shipped on Blue Ice
Molecular Weight
27,441 Da
3D Structure
ModBase 3D Structure for O54907
Source Note
The extracellular domain of mouse TWEAK (aa 94-249) is fused at the N-terminus to tenascin-C (TNC) (chicken) (aa 110-139) and a DYKDDDDK-tag
Endotoxin
<0.1EU/ug purified protein (LAL test)
Reconstitution
Reconstitute with 100ul sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein
Applications Tested/Suitable for TNC recombinant protein
ELISA (EIA)

Other Notes

Small volumes of TNC recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TNC purchase

MBS566260 is a recombinant protein targeting TNC. Also known as TNC:TWEAK, (soluble) (mouse), (recombinant). Host: HEK293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for TNC. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Tumor necrosis factor ligand superfamily member 12
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 12
NCBI Official Symbol
Tnfsf12
NCBI Official Synonym Symbols
Dr3l; Apo3l; Dr3lg; Tweak
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 12
UniProt Synonym Protein Names
TNF-related weak inducer of apoptosis; TWEAK
Protein Family
UniProt Gene Name
Tnfsf12
UniProt Synonym Gene Names
TWEAK
UniProt Entry Name
TNF12_MOUSE
NCBI Protein Information
tumor necrosis factor ligand superfamily member 12; TNF-related weak inducer of apoptosis

UniProt Comments for TNC

TNFSF12: Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines (By similarity)

Protein type: Cytokine; Motility/polarity/chemotaxis; Membrane protein, integral; Apoptosis

Cellular Component: extracellular space; membrane; perinuclear region of cytoplasm; plasma membrane; integral to membrane; extracellular region

Molecular Function: cytokine activity; tumor necrosis factor receptor binding

Biological Process: apoptosis; multicellular organismal development; positive regulation of protein catabolic process; immune response; angiogenesis; cell differentiation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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