Calcineurin (PPP3CA) Active Protein

Name: PPP3CA Active Protein; Calcineurin; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS566577
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS566577

MBS566577 is an active protein targeting PPP3CA. Also known as Calcineurin (human), (recombinant). Host: E Coli. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Calcineurin (PPP3CA), Active Protein

Full Product Name

Calcineurin (human), (recombinant)

Product Gene Name

PPP3CA active protein

Host

E Coli

Form/Format

Liquid. In 1X KI-128 Assay Buffer with increased DTT concentration (50mM TRIS, pH7.5, containing 100mM sodium chloride, 6mM MgCl2, 5mM DTT, 0.025% NP-40 and 0.5mM CaCl2)

Purity/Purification

>=90% (SDS-PAGE)

Concentration

100U/ul (lot specific)

Preparation and Storage

For long term storage, store at -80 degree C
Shipping: Shipped on Dry Ice

Molecular Weight

521

OMIM

114105

3D Structure

ModBase 3D Structure for Q08209

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Source Note

Human calcineurin Aalpha (MW 60kDa) and calcineurin B (MW 15kDa) heterodimer. Both subunits are coexpressed in a construct with yeast myristoyl-CoA:protein N-myristoyltransferase. The resulting highly active calcineurin (protein phosphatase 2B) is N-myristoylated on the CaNB subunit, similar to the native protein

Specific Activity

One unit= 1pmol/min using 0.15mM RII phosphopeptide substrate in the presence of calcium/calmodulin at 30 degree C. In the absence of calmodulin, the activity is <1%

Dry Ice Shipment

Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for PPP3CA active protein

Proteostasis; Posttranslational Modifications

Other Notes

Small volumes of PPP3CA active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PPP3CA. It may not necessarily be applicable to this product.

NCBI GI #

1352673

NCBI GeneID

5530

NCBI Accession #

Q08209.1

UniProt Primary Accession #

Q08209

UniProt Secondary Accession #

P63098

UniProt Related Accession #

Q08209

NCBI Official Full Name

Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

NCBI Official Synonym Full Names

protein phosphatase 3, catalytic subunit, alpha isozyme

NCBI Official Symbol

PPP3CA

NCBI Official Synonym Symbols

CALN; CCN1; CNA1; CALNA; PPP2B; CALNA1

UniProt Protein Name

Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

UniProt Synonym Protein Names

CAM-PRP catalytic subunit; Calmodulin-dependent calcineurin A subunit alpha isoform

Protein Family

Serine/threonine-protein phosphatase

UniProt Gene Name

PPP3CA

UniProt Synonym Gene Names

CALNA; CNA

UniProt Entry Name

PP2BA_HUMAN

NCBI Protein Information

serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; calcineurin A alpha; CAM-PRP catalytic subunit; calmodulin-dependent calcineurin A subunit alpha isoform; protein phosphatase 2B, catalytic subunit, alpha isoform; protein phosphatas

UniProt Comments for PPP3CA

PPP3CA: a calmodulin-dependent Ser/Thr phosphatase also known calcineurin A alpha. Involved in a wide range of biologic activities, acting as a Ca(2 )-dependent modifier of phosphorylation status. Dephosphorylates HSPB1. The calcineurin holoenzyme is composed of catalytic and regulatory subunits of 60 and 18 kD, respectively. .

Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; Z disc; cytosol; calcineurin complex; sarcolemma

Molecular Function: calmodulin binding; protein dimerization activity; protein binding; enzyme binding; calmodulin-dependent protein phosphatase activity; protein heterodimerization activity; protein serine/threonine phosphatase activity; drug binding; calcium ion binding

Biological Process: regulation of synaptic transmission; positive regulation of NFAT protein import into nucleus; T cell activation; response to amphetamine; skeletal muscle fiber development; protein amino acid dephosphorylation; negative regulation of insulin secretion; calcineurin-NFAT signaling pathway; transition between fast and slow fiber; dephosphorylation; protein import into nucleus; calcium ion transport; innate immune response; positive regulation of transcription from RNA polymerase II promoter; response to calcium ion; regulation of excitatory postsynaptic membrane potential; multicellular organismal response to stress; G1/S transition of mitotic cell cycle

Research Articles on PPP3CA

1. These findings therefore provide initial support for the novel mechanistic hypothesis that oxidation-induced global and/or local conformational changes within calcineurin

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PPP3CA active protein

Reference Product	PubMed Publications
PPP3R1 active protein	>7 publications with PPP3CA and PPP3R1
PPP2CB active protein	>3 publications with PPP3CA and PPP2CB
PPP2CA active protein	>3 publications with PPP3CA and PPP2CA
NFATC1 active protein	>2 publications with PPP3CA and NFATC1

Pathways associated with PPP3CA active protein

Products by Pathway	Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Alzheimers Disease Pathway antibodies	Alzheimers Disease Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram

Diseases associated with PPP3CA active protein

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>4 publications with PPP3CA and Nervous System Diseases
Brain Diseases Antibodies	>4 publications with PPP3CA and Brain Diseases
Hypertrophy Antibodies	>3 publications with PPP3CA and Hypertrophy
Liver Diseases Antibodies	>2 publications with PPP3CA and Liver Diseases
Inflammation Antibodies	>2 publications with PPP3CA and Inflammation
Hypertension Antibodies	>2 publications with PPP3CA and Hypertension
Carcinoma, Hepatocellular Antibodies	>2 publications with PPP3CA and Carcinoma, Hepatocellular
Kidney Diseases Antibodies	>1 publications with PPP3CA and Kidney Diseases
Edema Antibodies	>1 publications with PPP3CA and Edema
Necrosis Antibodies	>1 publications with PPP3CA and Necrosis

Organs/Tissues associated with PPP3CA active protein

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>10 publications with PPP3CA and Muscle
Blood Antibodies	>6 publications with PPP3CA and Blood
Brain Antibodies	>6 publications with PPP3CA and Brain
Heart Antibodies	>5 publications with PPP3CA and Heart
Vascular Antibodies	>4 publications with PPP3CA and Vascular
Kidney Antibodies	>2 publications with PPP3CA and Kidney
Bone Antibodies	>2 publications with PPP3CA and Bone
Liver Antibodies	>2 publications with PPP3CA and Liver
Eye Antibodies	>1 publications with PPP3CA and Eye
Mammary Gland Antibodies	>1 publications with PPP3CA and Mammary Gland