Coomassie-stained SDS-PAGE showing a timecourse of α1-antitrypsin (3ug) cleavage after incubation at 37°C with or without MMP-11 catalytic domain. Lanes are as follows: 1. Marker; 2. 1.5 ug α1-antitrypsin (0 hours); 3. 50 ng MMP-11, (0 hours); 4. 1 ug MMP-11, (0 hours); 5. 1.5 ug α1-antitrypsin incubated with 50 ng MMP-11 (0 hours); 6. 1.5 ug α1-antitrypsin incubated with 50 ng MMP-11 (3 hours); 7. 1.5 ug α1-antitrypsin incubated with 50 ng MMP-11 (8 hours); 8. 1.5 ug α1-antitrypsin incubated with 50 ng MMP-11 (24 hours); 9. 1.5 ug α1-antitrypsin incubated with 1 ug MMP-11 (0 hours); 10. 1.5 yg α1-antitrypsin incubated with 1 ug MMP-11 (24 hours); 11. 1.5 ug α1-antitrypsin (24 hours); 12. Marker.

MMP-11 Active Protein

Name: MMP-11 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS566607
Price: 785 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS566607

MBS566607 is an active protein targeting MMP-11. Also known as Matrix metalloproteinase 11, Stromelysin-3. Host: E Coli. MMP-11 may be a link between obesity and cancer. More details

$785 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MMP-11, Active Protein

Full Product Name

Matrix metalloproteinase 11, Stromelysin-3

Product Gene Name

MMP-11 active protein

Host

E Coli

Form/Format

Liquid. In 50mM TRIS, 5mM CaCl2, 300mM NaCl, 20uM ZnCl2, 0.5% Brij-35, and 30% glycerol

Purity/Purification

>95% (SDS-PAGE)
Purified by multi-step chromatography

Preparation and Storage

For long term storage, store at -80°C
Shipping: Shipped on Dry Ice

Molecular Weight

19.3kDa

OMIM

185261

3D Structure

ModBase 3D Structure for P24347

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Source Note

Produced in E. coli. Active Matrix Metalloproteinase-11 (MMP-11, Stromelysin-3) catalytic domain from human cDNA. The enzyme consists of the catalytic domain of human MMP-11 (Phe98-Ser266, NM_005940) with a C- terminal purification tag. MMPs lacking this domain cannot cleave native collagens; however, activity toward other targets such as gelatin, casein, or peptide substrates is unaffected. It may be an important link between obesity and cancer.

Specific Activity

Due to its unusual substrate preferences [A(A/Q)(N/A)~(L/Y)(T/V/M/R)(R/K), or G(G/A)E~LR5], MMP-11 cleaves MMP peptide substrates extremely slowly (several hours yield very little product). Therefore, the activity of each lot of MMP-11 is verified by digestion of macromolecules

Dry Ice Shipment

Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for MMP-11 active protein

Cellular Analysis; Cell Organelles & Structures; Proteostasis; Proteolysis & Protein Degradation

Application Notes for MMP-11 active protein

Useful to study enzyme kinetics, cleave target substrates, and screen for inhibitors.

Other Notes

Small volumes of MMP-11 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MMP-11. It may not necessarily be applicable to this product.

NCBI Accession #

NM_005940

UniProt Primary Accession #

P24347

UniProt Protein Name

Stromelysin-3

UniProt Synonym Protein Names

Matrix metalloproteinase-11

Protein Family

Stromelysin

UniProt Gene Name

MMP11

UniProt Synonym Gene Names

STMY3; SL-3; ST3; MMP-11

UniProt Entry Name

MMP11_HUMAN

UniProt Comments for MMP-11

MMP11: May play an important role in the progression of epithelial malignancies. Belongs to the peptidase M10A family.

Protein type: Extracellular matrix; EC 3.4.24.-; Secreted, signal peptide; Protease; Secreted

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: proteinaceous extracellular matrix; Golgi lumen; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity; calcium ion binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; collagen fibril organization; multicellular organismal development; proteolysis; negative regulation of fat cell differentiation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.