CDC25B Active Protein

Name: CDC25B Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS566633
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS566633

CDC25 protein tyrosine phosphatases activate cyclin dependent kinases by dephosphorylating them, promoting cell division. All three of the genes encoding the CDC25 phosphatases are considered potential oncogenes and the enzymes are drug discovery targets. In… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CDC25B, Active Protein

Full Product Name

CDC25B (human), (recombinant)

Product Gene Name

CDC25B active protein

Host

E Coli

Form/Format

Liquid. In 50mM TRIS/HCl, pH8.0, 150mM NaCl, 5mM DTT, 0.1mM EDTA, 0.03% Brij 35, 20% glycerol

Purity/Purification

Partially purified by single-step affinity chromatography and gel filtration

Preparation and Storage

For long term storage, store at -80 degree C
Shipping: Shipped on Dry Ice

Molecular Weight

65kDa

OMIM

116949

3D Structure

ModBase 3D Structure for P30305

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Source Note

Full length human CDC25B

Specific Activity

>=10U/mg assayed by 3-O-methyl fluorescein phosphate (OMFP) hydrolysis at pH8.2, 30 degree C. One unit is equal to 1 nmole phosphate hydrolyzed from OMFP per minute

Dry Ice Shipment

Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for CDC25B active protein

Cellular Analysis; Cell Growth & Development; Proteostasis; Posttranslational Modifications

Other Notes

Small volumes of CDC25B active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDC25B purchase

MBS566633 is an active protein targeting CDC25B. Also known as CDC25B (human), (recombinant). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CDC25B. It may not necessarily be applicable to this product.

NCBI Accession #

NM_021873

UniProt Primary Accession #

P30305

UniProt Protein Name

M-phase inducer phosphatase 2

UniProt Synonym Protein Names

Dual specificity phosphatase Cdc25B

UniProt Gene Name

CDC25B

UniProt Synonym Gene Names

CDC25HU2

UniProt Entry Name

MPIP2_HUMAN

UniProt Comments for CDC25B

CDC25B: a member of the MPI phosphatase family. Activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. Shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. At least four transcript variants for this gene exist. Three splice variant isoforms have been described.

Protein type: EC 3.1.3.48; Nuclear receptor co-regulator; Protein phosphatase, dual-specificity; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: nucleoplasm; spindle pole; centrosome; cytoplasm; cytosol

Molecular Function: protein binding; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: positive regulation of cytokinesis; mitosis; cell division; positive regulation of protein kinase activity; positive regulation of cell proliferation; mitotic cell cycle; G2/M transition of mitotic cell cycle; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; oocyte maturation; female meiosis I

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.