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Tyrosinase (TYR) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS566685

Tyrosinase is a copper-containing enzyme that catalyzes four steps in the melanin and catecholamine synthesis pathways. Like tyrosine hydroxylase (EC 1.13.16.2), it can catalyze the formation of L-DOPA in the brain and nervous system. It oxidizes monophenols,… More details

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Product Overview

Online Datasheet in PDF
Product Name
Tyrosinase, Active Protein
Full Product Name
Tyrosinase (human), (recombinant) (His-tag)
Product Gene Name
TYR active protein
Host
E Coli
Form/Format
Liquid. In 50mM TRIS/HCl, pH8.0, 1M NaCl, 0.05% Empigen BB, and 30% glycerol
Purity/Purification
>=85% (SDS-PAGE)
Purified by multi-step chromatography
Preparation and Storage
For long term storage, store at -80 degree C. Avoid freeze/thaw cycles
Shipping: Shipped on Dry Ice
Molecular Weight
~52kDa (SDS-PAGE)
Chromosome Location
Chromosome: 11; NC_000011.9 (88911040..89028927). Location: 11q14-q21
OMIM
103470
3D Structure
ModBase 3D Structure for P14679
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Activity
One microgram will produce greater than 90pmol of adduct at 37 degree C using 0.5mM L-Tyrosine as substrate in 50mM potassium phosphate, pH7.3, 2% dimethylformamide, 5mM MBTH (3-methyl-2-benzothiazolinone hydrazone) and monitoring formation of dopachrome-MBTH adduct at 484nm (epsilon484=22,300 M-1cm-1). Note: tyrosinases, in particular mammalian tyrosinase, are inherently inefficient enzymes, with typical changes in OD only in the deciOD range, although the reaction reaches maximal velocity quickly (typically within two minutes). In addition, its substrates and MBTH are very unstable; make stocks fresh and keep sealed in the dark until use. Lifetime and rate of enzymatic activity can be increased by the addition of CuCl to the assay buffer, but this will increase background and may complicate other redox reactions in the assay

Product Categories/Family for TYR active protein

Cellular Analysis; Cell Signaling
Application Notes for TYR active protein
Useful tool to study enzyme kinetics and screen for inhibitors.

SDS-PAGE Analysis of TYR active protein

Lane 1: MW Marker, Lane 2: 2 ug Tyrosinase.
TYR Active Protein – Cat# MBS566685

Tyrosinase Activity of TYR active protein

Formation of dopachrome-MBTH adduct monitoring at 484nm in a 96-well half area microwell plate with a range of enzyme loads. Maximum signal plateau was reached rapidly at 37 degree C, preventing an accurate time-dependent measurement.
TYR Active Protein, Tyrosinase Activity – Cat# MBS566685

Melanin and catecholamine synthesis pathways of TYR active protein

TYR Active Protein, Melanin and catecholamine synthesis pathways – Cat# MBS566685

Other Notes

Small volumes of TYR active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TYR purchase

MBS566685 is an active protein targeting TYR. Also known as Tyrosinase (human), (recombinant) (His-tag). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for TYR. It may not necessarily be applicable to this product.

NCBI GI #
4507753
NCBI GeneID
7299
NCBI Accession #
NP_000363.1
UniProt Primary Accession #
P14679
UniProt Secondary Accession #
Q15675; Q15676; Q15680; Q8TAK4; Q9BYY0; Q9BZX1
UniProt Related Accession #
P14679
GenBank Nucleotide #
NM_000372.4
NCBI Official Full Name
tyrosinase
NCBI Official Synonym Full Names
tyrosinase
NCBI Official Symbol
TYR
NCBI Official Synonym Symbols
CMM8; OCA1A; OCAIA; SHEP3
UniProt Protein Name
Tyrosinase
UniProt Synonym Protein Names
LB24-AB; Monophenol monooxygenase; SK29-AB; Tumor rejection antigen AB
Protein Family
Tyrosinase
UniProt Gene Name
TYR
UniProt Entry Name
TYRO_HUMAN
NCBI Protein Information
tyrosinase; LB24-AB; SK29-AB; monophenol monooxygenase; oculocutaneous albinism IA; tumor rejection antigen AB

NCBI Summary for TYR

The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene. [provided by RefSeq, Oct 2008]
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UniProt Comments for TYR

TYR: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone. Increased expression after UVB irradiation. Belongs to the tyrosinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.14.18.1; Cofactor and Vitamin Metabolism - riboflavin; Amino Acid Metabolism - tyrosine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q14.3

Cellular Component: perinuclear region of cytoplasm; lysosome; melanosome membrane; cytoplasm; melanosome; integral to membrane; Golgi-associated vesicle

Molecular Function: protein binding; protein homodimerization activity; copper ion binding; protein heterodimerization activity; monophenol monooxygenase activity

Biological Process: cell proliferation; visual perception; thymus development; melanin biosynthetic process from tyrosine; eye pigment biosynthetic process

Disease: Albinism, Ocular, With Sensorineural Deafness; Albinism, Oculocutaneous, Type Ib; Albinism, Oculocutaneous, Type Ia; Skin/hair/eye Pigmentation, Variation In, 3
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Research Articles on TYR

1. A G47D & 1379delTT mutation was seen in 4 members of an oculocutaneous albinism family. An unrelated patient was a compound heterozygote for the G47D & D42N mutations.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TYR active protein

 Reference Product  PubMed Publications
 TYRP1 active protein  >98 publications with TYR and TYRP1

Pathways associated with TYR active protein

 Products by Pathway  Pathway Diagram
 (S)-reticuline Biosynthesis II Pathway antibodies  (S)-reticuline Biosynthesis II Pathway Diagram
 (S)-reticuline Biosynthesis II Pathway antibodies  (S)-reticuline Biosynthesis II Pathway Diagram
 Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies  Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram
 Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies  Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram
 L-dopachrome Biosynthesis Pathway antibodies  L-dopachrome Biosynthesis Pathway Diagram
 L-dopachrome Biosynthesis Pathway antibodies  L-dopachrome Biosynthesis Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Riboflavin Metabolism Pathway antibodies  Riboflavin Metabolism Pathway Diagram
 Riboflavin Metabolism Pathway antibodies  Riboflavin Metabolism Pathway Diagram

Diseases associated with TYR active protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1870 publications with TYR and Neoplasms
 Nervous System Diseases Antibodies  >740 publications with TYR and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >389 publications with TYR and Cardiovascular Diseases
 Pain Antibodies  >378 publications with TYR and Pain
 Inflammation Antibodies  >331 publications with TYR and Inflammation
 Melanoma Antibodies  >312 publications with TYR and Melanoma
 Neoplasms, Experimental Antibodies  >264 publications with TYR and Neoplasms, Experimental
 Liver Diseases Antibodies  >200 publications with TYR and Liver Diseases
 Hypertension Antibodies  >190 publications with TYR and Hypertension
 Necrosis Antibodies  >185 publications with TYR and Necrosis

Organs/Tissues associated with TYR active protein

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >460 publications with TYR and Skin
 Eye Antibodies  >255 publications with TYR and Eye
 Thyroid Antibodies  >170 publications with TYR and Thyroid
 Connective Tissue Antibodies  >128 publications with TYR and Connective Tissue
 Mammary Gland Antibodies  >40 publications with TYR and Mammary Gland
 Ear Antibodies  >28 publications with TYR and Ear
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