UCH-L1 Active Protein

Name: UCH-L1 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS566741
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS566741

Ubiquitin C-terminal hydrolases (UCHs) are a family of cysteine hydrolases that catalyze the hydrolysis of amides, esters and thioesters of the C-terminus of ubiquitin. Mammalian neuronal cells abundantly express a deubiquitylating enzyme, ubiquitin… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

UCH-L1, Active Protein

Full Product Name

UCH-L1 (PGP9.5) (human), (recombinant) (His-tag)

Product Gene Name

UCH-L1 active protein

Host

E Coli

Form/Format

Liquid. In 50 mM TRIS, pH7.6, containing 1 mM dithiothreitol (DTT)

Preparation and Storage

For long term storage, store at -80 degree C
Shipping: Shipped on Dry Ice

Molecular Weight

24,824 Da

Chromosome Location

Chromosome: 4; NC_000004.11 (41258898..41270446). Location: 4p14

OMIM

191342

3D Structure

ModBase 3D Structure for P09936

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Specific Activity

>100 pmol/min/ug. Determined at 25 degree C with 1uM ubiquitin-AMC as substrate and UCH-L1

Dry Ice Shipment

Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for UCH-L1 active protein

Cellular Analysis; Stem Cells; Proteostasis; Protein Synthesis & Folding; Posttranslational Modifications; Ubiquitin & UBL Signaling

Other Notes

Small volumes of UCH-L1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UCH-L1 purchase

MBS566741 is an active protein targeting UCH-L1. Also known as UCH-L1 (PGP9.5) (human), (recombinant) (His-tag). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for UCH-L1. It may not necessarily be applicable to this product.

NCBI GI #

21361091

NCBI GeneID

7345

NCBI Accession #

NP_004172.2

UniProt Primary Accession #

P09936

UniProt Secondary Accession #

Q4W5K6; Q71UM0

UniProt Related Accession #

P09936

GenBank Nucleotide #

NM_004181.4

NCBI Official Full Name

ubiquitin carboxyl-terminal hydrolase isozyme L1

NCBI Official Synonym Full Names

ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)

NCBI Official Symbol

UCHL1

NCBI Official Synonym Symbols

PARK5; PGP95; PGP9.5; Uch-L1; PGP 9.5

UniProt Protein Name

Ubiquitin carboxyl-terminal hydrolase isozyme L1

UniProt Synonym Protein Names

Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1

Protein Family

Ubiquitin carboxyl-terminal hydrolase

UniProt Gene Name

UCHL1

UniProt Synonym Gene Names

UCH-L1; PGP9.5

UniProt Entry Name

UCHL1_HUMAN

NCBI Protein Information

ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin thioesterase L1; neuron cytoplasmic protein 9.5; ubiquitin C-terminal hydrolase

NCBI Summary for UCH-L1

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]

UniProt Comments for UCH-L1

UCHL1: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Monomer. Homodimer. Interacts with SNCA. Interacts with COPS5. Found in neuronal cell bodies and processes throughout the neocortex. Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. Belongs to the peptidase C12 family.

Protein type: Cell development/differentiation; EC 3.4.19.12; Ligase; Protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; cell soma; axon; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; omega peptidase activity; ubiquitin binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; alpha-2A adrenergic receptor binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; cell proliferation; negative regulation of MAP kinase activity; protein deubiquitination; axon transport of mitochondrion; axon target recognition; eating behavior; neuromuscular process; sensory perception of pain; muscle fiber development; adult walking behavior

Disease: Neurodegeneration With Optic Atrophy, Childhood-onset; Parkinson Disease 5, Autosomal Dominant

Research Articles on UCH-L1

1. Results showed that NT, NPY, and PGP 9.5 neurofibers are almost equally present in pseudocapsule of uterine fibroids as in normal myometrium of a no pregnant uterus. As all of these neuropeptides are present in the uterine muscle and can affect muscle contractility, uterine peristalsis and muscular healing.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with UCH-L1 active protein

Reference Product	PubMed Publications
HSP90AA1 active protein	>2 publications with UCH-L1 and HSP90AA1
CDKN1B active protein	>2 publications with UCH-L1 and CDKN1B
NEDD8 active protein	>1 publications with UCH-L1 and NEDD8
LAMP2 active protein	>1 publications with UCH-L1 and LAMP2
HSPA8 active protein	>1 publications with UCH-L1 and HSPA8

Pathways associated with UCH-L1 active protein

Products by Pathway	Pathway Diagram
Alpha-synuclein Signaling Pathway antibodies	Alpha-synuclein Signaling Pathway Diagram
Parkinson's Disease Pathway antibodies	Parkinson's Disease Pathway Diagram
Parkinsons Disease Pathway antibodies	Parkinsons Disease Pathway Diagram
Proteasome Degradation Pathway antibodies	Proteasome Degradation Pathway Diagram

Diseases associated with UCH-L1 active protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>263 publications with UCH-L1 and Neoplasms
Nervous System Diseases Antibodies	>192 publications with UCH-L1 and Nervous System Diseases
Brain Diseases Antibodies	>142 publications with UCH-L1 and Brain Diseases
Inflammation Antibodies	>46 publications with UCH-L1 and Inflammation
Adenocarcinoma Antibodies	>29 publications with UCH-L1 and Adenocarcinoma
Peripheral Nervous System Diseases Antibodies	>29 publications with UCH-L1 and Peripheral Nervous System Diseases
Cardiovascular Diseases Antibodies	>27 publications with UCH-L1 and Cardiovascular Diseases
Disease Models, Animal Antibodies	>27 publications with UCH-L1 and Disease Models, Animal
Necrosis Antibodies	>19 publications with UCH-L1 and Necrosis
Nerve Degeneration Antibodies	>18 publications with UCH-L1 and Nerve Degeneration

Organs/Tissues associated with UCH-L1 active protein

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>173 publications with UCH-L1 and Nerve
Brain Antibodies	>106 publications with UCH-L1 and Brain
Skin Antibodies	>85 publications with UCH-L1 and Skin
Bone Antibodies	>59 publications with UCH-L1 and Bone
Lymph Antibodies	>55 publications with UCH-L1 and Lymph
Lung Antibodies	>36 publications with UCH-L1 and Lung
Mouth Antibodies	>27 publications with UCH-L1 and Mouth
Testis Antibodies	>20 publications with UCH-L1 and Testis
Uterus Antibodies	>19 publications with UCH-L1 and Uterus
Embryonic Tissue Antibodies	>18 publications with UCH-L1 and Embryonic Tissue