DKK-1 Active Protein

Name: DKK-1 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS566756
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS566756

Dickkopf-related protein 1 (Dkk-1), which belongs to the dickkopf family, is a secreted protein with two cysteine rich domains and is involved in embryonic development and many human diseases. The regulation of Wnt signaling and Dkk1 inhibition has… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Dkk-1, Active Protein

Full Product Name

Dkk-1 (mouse), (recombinant)

Product Gene Name

Dkk-1 active protein

Host

Cell culture

Form/Format

Lyophilized from a solution containing 1X PBS, 1% CHAPS, and 0.1% BSA

Purity/Purification

>75% by SDS-PAGE; >75% by HPLC analyses

Preparation and Storage

For long term storage, store at -80 degree C
Stable for at least 12 months after receipt when stored, as supplied, at -80 degree C. Following reconstitution, stable for three months when stored at or below -20 degree C
Shipping: Shipped on Dry Ice

Molecular Weight

29,298 Da

3D Structure

ModBase 3D Structure for O54908

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Endotoxin

Less than 0.2 ng perug/ (2EU/ug)

Biological Activity

Wnt-3a can induce beta-catenin mediated response in a TCF/LEF reporter gene luciferase assay

Reconstitution

Prepare 5ug/mL stock solution of Wnt3a by adding 200uL deionized water into the vial of lyophilized Wnt3a

Product Categories/Family for Dkk-1 active protein

Cellular Analysis; Cell Signaling; Stem Cells

Other Notes

Small volumes of Dkk-1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Dkk-1 purchase

MBS566756 is an active protein targeting Dkk-1. Also known as Dkk-1 (mouse), (recombinant). Host: Cell culture.

Additional Details

NCBI/Uniprot data below describe general gene information for Dkk-1. It may not necessarily be applicable to this product.

NCBI GI #

408360053

NCBI GeneID

13380

NCBI Accession #

O54908.2

UniProt Primary Accession #

O54908

UniProt Secondary Accession #

Q80UL5

UniProt Related Accession #

O54908

NCBI Official Full Name

Dickkopf-related protein 1

NCBI Official Synonym Full Names

dickkopf homolog 1 (Xenopus laevis)

NCBI Official Symbol

Dkk1

NCBI Official Synonym Symbols

mdkk-1

UniProt Protein Name

Dickkopf-related protein 1

Protein Family

Dickkopf-related protein

UniProt Gene Name

Dkk1

UniProt Synonym Gene Names

Dickkopf-1; Dkk-1; mDkk-1

UniProt Entry Name

DKK1_MOUSE

NCBI Protein Information

dickkopf-related protein 1; dkk-1; dickkopf-1; dickkopf-like protein 1

UniProt Comments for Dkk-1

DKK1: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP6. Interacts (via the C-termianl Cys- rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt- mediated signaling. Placenta. Belongs to the dickkopf family.

Protein type: Secreted; Secreted, signal peptide; Inhibitor

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: receptor antagonist activity; protein binding; low-density lipoprotein receptor binding

Biological Process: cellular morphogenesis during differentiation; negative regulation of skeletal muscle development; Wnt receptor signaling pathway; response to retinoic acid; negative regulation of Wnt receptor signaling pathway; multicellular organismal development; regulation of receptor internalization; regulation of Wnt receptor signaling pathway; negative regulation of peptidyl-serine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of BMP signaling pathway; mesoderm formation; hair follicle development; negative regulation of mesodermal cell fate specification; endoderm formation; forebrain development; regulation of endodermal cell fate specification; negative regulation of protein complex assembly; endoderm development; embryonic limb morphogenesis

Research Articles on Dkk-1

1. extra-follicular modulators Bmp2, Dkk1, and Sfrp4 increase in early anagen.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Dkk-1 active protein

Reference Product	PubMed Publications
Ctnnb1 active protein	>478 publications with Dkk-1 and Ctnnb1
Wnt3a active protein	>126 publications with Dkk-1 and Wnt3a
Lrp5 active protein	>116 publications with Dkk-1 and Lrp5
Lrp6 active protein	>102 publications with Dkk-1 and Lrp6
Sfrp2 active protein	>40 publications with Dkk-1 and Sfrp2
Wnt1 active protein	>38 publications with Dkk-1 and Wnt1
Kremen1 active protein	>18 publications with Dkk-1 and Kremen1
Msx2 active protein	>11 publications with Dkk-1 and Msx2
Sp7 active protein	>3 publications with Dkk-1 and Sp7

Pathways associated with Dkk-1 active protein

Products by Pathway	Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
PluriNetWork Pathway antibodies	PluriNetWork Pathway Diagram
RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway antibodies	RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By WNT In Cancer Pathway antibodies	Signaling By WNT In Cancer Pathway Diagram
Signaling By Wnt Pathway antibodies	Signaling By Wnt Pathway Diagram
TCF Dependent Signaling In Response To WNT Pathway antibodies	TCF Dependent Signaling In Response To WNT Pathway Diagram
Wnt Signaling Pathway NetPath antibodies	Wnt Signaling Pathway NetPath Diagram
Wnt Signaling Pathway antibodies	Wnt Signaling Pathway Diagram
Wnt Signaling Pathway antibodies	Wnt Signaling Pathway Diagram

Diseases associated with Dkk-1 active protein

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>87 publications with Dkk-1 and Cardiovascular Diseases
Nervous System Diseases Antibodies	>40 publications with Dkk-1 and Nervous System Diseases
Adenocarcinoma Antibodies	>38 publications with Dkk-1 and Adenocarcinoma
Inflammation Antibodies	>37 publications with Dkk-1 and Inflammation
Brain Diseases Antibodies	>36 publications with Dkk-1 and Brain Diseases
Necrosis Antibodies	>34 publications with Dkk-1 and Necrosis
Breast Neoplasms Antibodies	>34 publications with Dkk-1 and Breast Neoplasms
Carcinoma, Hepatocellular Antibodies	>32 publications with Dkk-1 and Carcinoma, Hepatocellular
Fractures, Bone Antibodies	>31 publications with Dkk-1 and Fractures, Bone
Neoplasm Metastasis Antibodies	>30 publications with Dkk-1 and Neoplasm Metastasis

Organs/Tissues associated with Dkk-1 active protein

Organ/Tissue Name	Pubmed Publications
Embryonic Tissue Antibodies	>101 publications with Dkk-1 and Embryonic Tissue
Bone Marrow Antibodies	>99 publications with Dkk-1 and Bone Marrow
Brain Antibodies	>78 publications with Dkk-1 and Brain
Kidney Antibodies	>43 publications with Dkk-1 and Kidney
Eye Antibodies	>42 publications with Dkk-1 and Eye
Joint Antibodies	>40 publications with Dkk-1 and Joint
Extraembryonic Tissue Antibodies	>2 publications with Dkk-1 and Extraembryonic Tissue