Histone H1 (H1) Antibody – Mouse Monoclonal

Monoclonal

IgG1

r1415-1

Mouse

Human, Mouse, Rat. Others not known.

200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 1mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.

~30kDa

ModBase 3D Structure for P14136

Nuclei of human leukemia biopsy cells

Nuclear

3q21-q22; 6q21-q22; 22q13.1

HeLa, A-431, LNCap or Jurkat cells.Breast carcinoma.