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Carcinoembryonic Antigen / CEA Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS570075

This monoclonal antibody is reactive with Human carcinoembryonic antigen (CEA), a tumour associated antigen with oncofetal characteristics. Although CEA can be found in tissues of non-neoplastic diseases and normal epithelia, it occurs also in a large variety… More details

$605 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Carcinoembryonic Antigen / CEA, Monoclonal Antibody
Full Product Name
Mouse anti Carcinoembryonic Antigen / CEA
Clonality
Monoclonal
Isotype
IgG1
Clone Number
PARLAM 4
Host
Mouse
Species Reactivity
Human
Form/Format
Each vial contains 100 ug 1 mg/ml purified antibody in PBS + 0.09% sodium azide.
Specificity
Most polyclonal CEA antisera show cross-reactivity with related antigens such as biliary glycoprotein (BGP) and non-specific cross-reacting antigen 1/11 (NCA). PARLAM 4 does not show cross reactivity, neither with BGP nor with NCA. In immunoblotting the antibody recognizes a single band of 180 kD.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Source Note
PARLAM 4 is a Mouse monoclonal IgG1 antibody derived by fusion of Sp 2/0 Ag 14 Mouse myeloma cells with spleen cells from a BABL/c Mouse immunized with isolated Human CEA. The immunogen has been isolated from Human colonic carcinoma cells.
Applications Tested/Suitable for anti-Carcinoembryonic Antigen / CEA antibody
Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
PARLAM 4 is useful for flow cytometry, immunoblotting, immunocytochemistry on methanol fixed cells and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. The antibody is also reactive in formalin-fixed and paraffin-embedded tissue sections after treatment with citRate buffer pH 6.0 in an autoclave. Human colon carcinoma tissue is used as positive control. Optimal antibody dilution should be determined by titRation; we recommend a 1:25 - 1:100 dilution for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:500 for immunoblotting appliCation.

Product References and Citations for anti-Carcinoembryonic Antigen / CEA antibody

Verstijnen CP, Arends JW, Moerkerk PT, Warnaar S, Hilgers J, Bosman FT (1986). CEA-specificity of CEA-reactive monoclonal antibodies. Immuno-chemical and immunocytochemical studies. Anticancer Research, 6, 97-104. Henzen-Logmans SC, Schipper NW, Poels LG, Stolk K, Kenemans P, Meyer CJ. (1988) Use of statistical evaluation of antigen profiles in differential diagnosis between colonic and ovarian adenocarcinomas. J.Clin Pathol. 41:644-9. Taal BG, Hageman PC, Delemarre JF, Bonfrèr JM, den Hartog Jager FC. (1992) Metastatic ovarian or colonic cancer: a clinical challenge. Eur J Cancer. 28: 394-9.

Other Notes

Small volumes of anti-Carcinoembryonic Antigen / CEA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Carcinoembryonic Antigen / CEA purchase

MBS570075 is an antibody targeting Carcinoembryonic Antigen / CEA. Also known as Mouse anti Carcinoembryonic Antigen / CEA. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Carcinoembryonic Antigen / CEA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
carcinoembryonic antigen, partial

UniProt Comments for Carcinoembryonic Antigen / CEA

CEACAM5: Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. Belongs to the immunoglobulin superfamily. CEA family.

Protein type: Immunoglobulin superfamily; Membrane protein, integral; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 19q13.1-q13.2

Cellular Component: integral to plasma membrane; basolateral plasma membrane

Molecular Function: identical protein binding; protein homodimerization activity

Biological Process: homotypic cell-cell adhesion; negative regulation of apoptosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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