Rat Anti-GPC3 Monoclonal Antibody – Cat# MBS570091

Figure 1: Immunohistochemistry on frozen section of Human kidney showing strong reactivity in the extracellular matrix and basement membrane.

Heparan Sulphate Proteoglycan (GPC3) Antibody – Rat Monoclonal

Name: GPC3 Antibody; Monoclonal; Rat; Bovine/Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS570091
Price: 605 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS570091

Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulphate, heparin, chondroitin sulphate, dermatan sulphate or keRatan sulphate). At… More details

$605 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Heparan Sulphate Proteoglycan (GPC3), Monoclonal Antibody

Full Product Name

Rat anti Heparan Sulphate Proteoglycan

Product Gene Name

anti-GPC3 antibody

Clonality

Monoclonal

Isotype

IgG2a

Clone Number

A7L6

Host

Rat

Species Reactivity

Bovine, Human, Mouse, Rat, Cod and Wolffish.

Form/Format

The vial contains 100 ul 1 mg/ml monoclonal purified antibody in PBS containing 0.09% sodium azide.

Specificity

A7L6 recognizes domain IV of the core protein of the large heparan sulphate proteoglycan or perlecan. The reactivity is independent of the galactosaminoglycan moieties. Therefore, the epitope is not sensitive to heparitinase treatment.

Preparation and Storage

The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Chromosome Location

chromosome: X; Location: Xq26.1

OMIM

300037

3D Structure

ModBase 3D Structure for P51654

Source Note

A7L6 is a Rat monoclonal IgG2a antibody derived by fusion of X63 Ag8.653 Mouse myeloma cells with spleen cells from a Fisher Rat immunized with high molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.

Warning and Caution

This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.

Product Categories/Family for anti-GPC3 antibody

Extracellular matrix

Applications Tested/Suitable for anti-GPC3 antibody

Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Immunoprecipitation (IP), Western Blot (WB)

Application Notes for anti-GPC3 antibody

A7L6 is useful for immunoprecipitation, immunoblotting, immunocytochemistry and immuno-histochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Product References and Citations for anti-GPC3 antibody

Couchman, J.R. (1987). Heterogeneous distribution of a basement membrane heparin sulfate proteoglycan in Rat tissues. The Journal of Cell Biology 105, 1901-16. Couchman, J. R., and Ljubimov, A. V. (1989). Mammalian tissue distribution of a large heparan sulfate proteoglycan detected by monoclonal antibodies. Matrix 9, 311-21. 3. Horiguchi, Y., Couchman, J. R., Ljubimov, A. V., Yamasaki, H., and Fine, J. D. (1989). Distribution, ultrastructural localization, and ontogeny of the core protein of a heparan sulfate proteoglycan in Human skin and other basement membranes. J Histochem Cytochem 37, 961-70. Ljubimov, A. V., Bartek, J., Couchman, J. R., Kapuller, L. L., Veselov, V. V., Kovarik, J., Perevoshchikov, A. G., and Krutovskikh, V. A. (1992). Distribution of individual components of basement membrane in Human colon polyps and adenocarcinomas as revealed by monoclonal antibodies. Int J Cancer 50, 562-66. Couchman, J. R., Ljubimov, A. V., Sthanam, M., Horchar, T., and Hassell, J. R. (1995). Antibody mapping and tissue localization of globular and cysteine-rich regions of perlecan domain III. J Histochem Cytochem 43, 955-63. Tapanadechopone, P., Hassell, J. R., Rigatti, B., and Couchman, J. R. (1999). Localization of glycosaminoglycan substitution sites on domain V of Mouse perlecan. Biochem Biophys Res Commun 265, 680-90. Tingbø, M. G., Kolset, S. O., Ofstad, R., Enersen, G., Hannesson, K. O. (2006). IdentifiCation and distribution of heparan sulfate proteoglycans in the white muscle of Atlantic cod (Gadus morhua) and spotted wolffish (Anarhichas minor). CompaRative Biochemistry and Physiology Part B 143,441-52.

Other Notes

Small volumes of anti-GPC3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GPC3 purchase

MBS570091 is an antibody targeting GPC3. Also known as Rat anti Heparan Sulphate Proteoglycan. Reactive in Bovine, Human, Mouse, Rat, Cod and Wolffish.. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for GPC3. It may not necessarily be applicable to this product.

NCBI GI #

1245417

NCBI GeneID

2719

UniProt Primary Accession #

P51654

UniProt Related Accession #

P51654; Q2L880; Q2L882; Q53H15; Q8IYG2

NCBI Official Full Name

heparan sulphate proteoglycan

NCBI Official Synonym Full Names

glypican 3

NCBI Official Symbol

GPC3

NCBI Official Synonym Symbols

SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2; GPC3

UniProt Protein Name

Glypican-3

UniProt Synonym Protein Names

GTR2-2; Intestinal protein OCI-5; MXR7

Protein Family

Glypican

UniProt Gene Name

GPC3

UniProt Synonym Gene Names

OCI5

UniProt Entry Name

GPC3_HUMAN

NCBI Protein Information

glypican-3; secreted glypican-3; glypican proteoglycan 3; intestinal protein OCI-5; heparan sulphate proteoglycan

NCBI Summary for GPC3

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

UniProt Comments for GPC3

GPC3: Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1); also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. Belongs to the glypican family.

Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xq26.1

Cellular Component: lysosomal lumen; proteinaceous extracellular matrix; extracellular space; anchored to plasma membrane; integral to plasma membrane; Golgi lumen; plasma membrane

Molecular Function: heparan sulfate proteoglycan binding; protein binding

Biological Process: phototransduction, visible light; anatomical structure morphogenesis; glycosaminoglycan metabolic process; negative regulation of peptidase activity; pathogenesis; positive regulation of endocytosis; osteoclast differentiation; embryonic hindlimb morphogenesis; body morphogenesis; bone mineralization; chondroitin sulfate metabolic process; positive regulation of glucose import; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; ureteric bud branching; negative regulation of smoothened signaling pathway; carbohydrate metabolic process; positive regulation of protein catabolic process; positive regulation of smoothened signaling pathway; retinoid metabolic process; positive regulation of BMP signaling pathway; lung development; negative regulation of epithelial cell proliferation; anterior/posterior axis specification; negative regulation of growth

Disease: Simpson-golabi-behmel Syndrome, Type 1; Wilms Tumor 1

Research Articles on GPC3

1. SALL4 is a more sensitive marker than PLAP, AFP, or glypican-3 for extragonadal yolk sac tumors.
2. Data show that mutated GPC3 lacking the GPI anchoring domain (sGPC3) significantly inhibited the in vivo growth, blocked Wnt signaling and Erk1/2 and Akt phosphorylation in tumors.
3. Our data supports the potentially significant diagnostic utility of GPC-3 in fine-needle aspiration biopsies in differentiating primary malignant from benign and preneoplastic liver lesions, and metastatic carcinomas.
4. Report the expression profile of glypican-3 and its relation to macrophage population in human hepatocellular carcinoma.
5. GPC3 expression is correlated with poor prognosis in hepatocellular carcinoma.
6. overexpression of glypican-3 may be related to the development and aggressive behavior of ovarian clear cell adenocarcinoma.
7. Detection of glypican-3-specific CTLs in chronic hepatitis and liver cirrhosis.
8. A reliable marker for the detection of hepatocellular carcinoma in liver biopsies.
9. A patient with the Simpson-Golabi-Behmel syndrome displays a loss-of-function point mutation in GPC3 that inhibits the attachment of this proteoglycan to the cell surface.
10. GPC3 is frequently expressed in noncutaneous small cell neuroendocrine carcinoma of various origins, in particular in Merkel cell carcinoma, which, in combination with CK20
11. The effector cells stimulated with DCs that were transfected with pEF-hGPC3 plasmid could effectively lyse GPC3 expressing HepG2 cells.
12. GPC3 expression in HCCs did not correlate with the size, differentiation, or stage of the tumors; the presence or absence of cirrhotic background; or the underlying etiologies
13. Report expression pattern of glypican-3 (GPC3) during human embryonic and fetal development.
14. Glypican-3 expression is a potential candidate marker for early detection of lung squamous cell carcinoma.
15. Glypican-3 appears to be a reliable markers for ovarian germ-cell tumors.
16. GPC3 confers oncogenecity through the interaction between IGF-II and its receptor, and the subsequent activation of the IGF-signaling pathway.
17. GPC3 is expressed at an early stage, suggesting that GPC3 expression in thyroid cancer is an early event in developing papillary carcinoma.
18. staining for glypican-3 is a highly sensitive and specific method capable of distinguishing HCC from other benign and malignant hepatic lesions
19. GPC3 seems to be a promising diagnostic marker for differentiating yolk sac tumors from ovarian clear cell carcinomas.
20. GPC3 may play a role in the tumorigenesis of hepatoblastoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GPC3 antibody

Reference Product	PubMed Publications
IGF2 antibody	>8 publications with GPC3 and IGF2
FGF2 antibody	>5 publications with GPC3 and FGF2
WNT3A antibody	>1 publications with GPC3 and WNT3A

Pathways associated with anti-GPC3 antibody

Products by Pathway	Pathway Diagram
A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway antibodies	A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway Diagram
Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway antibodies	Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway Diagram
Glycosaminoglycan Metabolism Pathway antibodies	Glycosaminoglycan Metabolism Pathway Diagram
Glypican 3 Network Pathway antibodies	Glypican 3 Network Pathway Diagram
Glypican Pathway antibodies	Glypican Pathway Diagram
HS-GAG Biosynthesis Pathway antibodies	HS-GAG Biosynthesis Pathway Diagram
HS-GAG Degradation Pathway antibodies	HS-GAG Degradation Pathway Diagram
Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway antibodies	Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Carbohydrates Pathway antibodies	Metabolism Of Carbohydrates Pathway Diagram

Diseases associated with anti-GPC3 antibody

Disease Name	Pubmed Publications
Carcinoma, Hepatocellular Antibodies	>105 publications with GPC3 and Carcinoma, Hepatocellular
Adenocarcinoma Antibodies	>95 publications with GPC3 and Adenocarcinoma
Liver Neoplasms Antibodies	>92 publications with GPC3 and Liver Neoplasms
Liver Diseases Antibodies	>90 publications with GPC3 and Liver Diseases
Hepatitis Antibodies	>22 publications with GPC3 and Hepatitis
Liver Cirrhosis Antibodies	>20 publications with GPC3 and Liver Cirrhosis
Genetic Diseases, Inborn Antibodies	>20 publications with GPC3 and Genetic Diseases, Inborn
Wilms Tumor Antibodies	>15 publications with GPC3 and Wilms Tumor
Adenoma Antibodies	>14 publications with GPC3 and Adenoma
Kidney Diseases Antibodies	>10 publications with GPC3 and Kidney Diseases

Organs/Tissues associated with anti-GPC3 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>102 publications with GPC3 and Liver
Blood Antibodies	>41 publications with GPC3 and Blood
Kidney Antibodies	>21 publications with GPC3 and Kidney
Bone Antibodies	>19 publications with GPC3 and Bone
Embryonic Tissue Antibodies	>19 publications with GPC3 and Embryonic Tissue
Lung Antibodies	>12 publications with GPC3 and Lung
Heart Antibodies	>11 publications with GPC3 and Heart
Ovary Antibodies	>8 publications with GPC3 and Ovary
Placenta Antibodies	>7 publications with GPC3 and Placenta
Vascular Antibodies	>6 publications with GPC3 and Vascular