Mouse Anti-Collagen Type IV Monoclonal Antibody – Cat# MBS570107

Figure 1. Strong collagen type IV specific staining of MBS570107 Clone 1043) in the basement membrane surrounding epithelial ducts and glomeruli in a frozen section of human kidney up to a 1:200 dilution. Also staining in the connective tissue and muscular layer of blood vessels. No staining of epithelial cells.

Collagen Type IV Antibody – Mouse Monoclonal

Name: Collagen Type IV Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS570107
Price: 605 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS570107

The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasma. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. In… More details

$605 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Collagen Type IV, Monoclonal Antibody

Full Product Name

Mouse anti Collagen Type IV

Clonality

Monoclonal

Isotype

IgG1

Clone Number

1043

Host

Mouse

Species Reactivity

Human
Does not react with other species

Form/Format

Each vial contains 100 ul 1mg/ml of purified monoclonal antibody in PBS containing 0.09% sodium azide

Specificity

1043 recognizes type IV collagen in the extracellular - basement membranes of normal and pathological human tissues.

Preparation and Storage

The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Source Note

1043 is a Mouse monoclonal IgG1 antibody derived by fusion of Sp 2/0 Mouse myeloma cells with spleen cells from a BABL/c Mouse immunized with purified Human type IV collagen. The immunogen has been isolated from Human placenta.

Product Categories/Family for anti-Collagen Type IV antibody

Cancer

Applications Tested/Suitable for anti-Collagen Type IV antibody

Flow Cytometry (FC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)

Application Notes for anti-Collagen Type IV antibody

1043 is useful for immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2 . For immunohistochemistry on paraffin sections the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature. Optimal antibody dilution should be determined by titration; 1:50 - 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications

Product References and Citations for anti-Collagen Type IV antibody

Visser, R., van der Beek, J.M.H., Havenith, M.G., Cleutjens, J.P.M. and Bosman, F.T. (1985). Immunocytochemical detection of basement membrane antigens in the histopathological evaluation of laryngeal dysplasia and neoplasia. Histopathology 10, 171-180. Cleutjens, J.P.M., Havenith, M.G., Kasper, M., Vallinga, M., and Bosman, F.T. (1990). Absence of type IV collagen in the centre of the corneal epithelial basement membrane. Histochemical Journal 22, 688-94. Havenith, M.G. et al. (1987). Human specific anti-type IV collagen monoclonal antibodies, characterisation and immunohistochemical appliCation. Histochemistry 87, 123-28. Schapers, R.F.M., Pauwels, R.P.E., Havenith, M.G., Smeets, A.W.G.B, van den Brandt and P.A. Bosman, F.T. (1990). Prognostic Significance of type IV collagen and laminin immunoreactivity in urothelial carcinomas of the bladder. Cancer 66, 2583-88.

Other Notes

Small volumes of anti-Collagen Type IV antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Collagen Type IV purchase

MBS570107 is an antibody targeting Collagen Type IV. Also known as Mouse anti Collagen Type IV. Reactive in Human
Does not react with other species. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Collagen Type IV. It may not necessarily be applicable to this product.

Protein Family

Collagen type IV alpha-3-binding protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.