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Mitotic Cells Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS570109

The life cycle of a eukaryotic cell consists of various phases, two of which can morphologically and biochemically be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation and… More details

$605 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Mitotic cells, Monoclonal Antibody
Full Product Name
Mouse anti Mitotic cells
Clonality
Monoclonal
Isotype
IgM
Clone Number
8B3G
Host
Mouse
Species Reactivity
Human, Zebrafish
Form/Format
Each vial contains 100 ul purified monoclonal antibody in PBS containing 0.09% sodium azide.
Specificity
8B3G strongly stains mitotic cells and can therefore be used in flow cytometric analyses of cell suspensions to detect the mitotic index. Together with a quantitative DNA staining procedure (e.g. propidium iodide) 8B3G clearly distinguishes these M-phase cells from cell at other stages of the cell cycle (see figure). Dynamic information can be obtained by combining BrdU incorpoRation with 8B3G staining, which can distinguish and quantitate the four major fractions of the cell cycle.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Source Note
8B3G is a Mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Mouse immunized with a total cell lysate of the Human bladder carcinoma cell line T24.
Applications Tested/Suitable for anti-Mitotic cells antibody
Flow Cytometry (FC), Immunocytochemistry (IHC)
Application Notes for anti-Mitotic cells antibody
8B3G can be used for flow cytometric analyses and immunocytochemistry. 8B3G is not suitable for immunoblotting. Optimal antibody dilution should be determined by titRation; recommended range is 1:50 - 1:100 for flow cytometry, and for immunocytochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent.

Testing Data of anti-Mitotic cells antibody

Figure 1: Immunofluorescence staining of a 7 days old zebrafish embryo
Mouse Anti-Mitotic Cells Monoclonal Antibody – Cat# MBS570109

Product References and Citations for anti-Mitotic cells antibody

1. No references available

Other Notes

Small volumes of anti-Mitotic cells antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Mitotic cells purchase

MBS570109 is an antibody targeting Mitotic cells. Also known as Mouse anti Mitotic cells. Reactive in Human, Zebrafish. Host: Mouse.
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