Rat Anti-NID1 Monoclonal Antibody – Cat# MBS570112

Entactin / Nidogen (NID1) Antibody – Rat Monoclonal

Name: NID1 Antibody; Monoclonal; Rat; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS570112
Price: 605 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS570112

Basement membrane components play an important role in cell adhesion, locomotion, differentiation and embryonic compartmentalization. Although the precise composition and structure of basal laminae varies from tissue to tissue, as well as within the same… More details

$605 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Entactin / NiDogen (Nid1), Monoclonal Antibody

Full Product Name

Rat anti Entactin / NiDogen

Product Gene Name

anti-Nid1 antibody

Clonality

Monoclonal

Isotype

IgG2a

Clone Number

ELM1

Host

Rat

Species Reactivity

Mouse

Form/Format

Each vial contains 100 ul 1.0 mg/ml purified monoclonal antibody in PBS containing 50% glycerol, 0.1% BSA and 0.02% sodium azide.

Specificity

ELM1 reacts with entactin.

Preparation and Storage

Store at 4 degree C, or in small aliquots at -20 degree C.

Molecular Weight

136,538 Da

Chromosome Location

chromosome: 13; Location: 13 A1; 13 7.0 cM

3D Structure

ModBase 3D Structure for P10493

Source Note

ELM1 is a Rat monoclonal IgG2a antibody derived by fusion of X63 Ag 8.653 Mouse myeloma cells with spleen cells from a Fisher Rat immunized with a partially purified prepaRation of laminin from the EHS Mouse tumor.

Product Categories/Family for anti-Nid1 antibody

Cell adhesion; Extracellular matrix; Cell adhesion

Applications Tested/Suitable for anti-Nid1 antibody

Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Western Blot (WB)

Application Notes for anti-Nid1 antibody

ELM1 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titRation; recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.

Product References and Citations for anti-Nid1 antibody

Ljubimov, A. V., Afanasjeva, A. V., Litvinova, L. V., Senin, V.M. (1986). Basement membrane components produced by a Mouse ascites teRatocarcinoma TB24, Exp Cell Res 165, 530-40. Couchman, J. R., and Ljubimov, A. V. (1989). Mammalian tissue distribution of a large heparan sulfate proteoglycan detected by monoclonal antibodies. Matrix 9, 311-321.

Other Notes

Small volumes of anti-Nid1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Nid1 purchase

MBS570112 is an antibody targeting Nid1. Also known as Rat anti Entactin / NiDogen. Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for Nid1. It may not necessarily be applicable to this product.

NCBI GI #

995952

NCBI GeneID

18073

UniProt Primary Accession #

P10493

UniProt Secondary Accession #

Q3TKX9; Q8BQI3; Q8C3U8; Q8C9P6

UniProt Related Accession #

P10493; Q3TKX9; Q99N24

NCBI Official Full Name

entactin

NCBI Official Synonym Full Names

nidogen 1

NCBI Official Symbol

Nid1

NCBI Official Synonym Symbols

Nid; entactin; nidogen-1; A630025O17; entactin-1; Nid1

UniProt Protein Name

Nidogen-1

UniProt Synonym Protein Names

Entactin

Protein Family

Nidogen

UniProt Gene Name

Nid1

UniProt Synonym Gene Names

Ent

UniProt Entry Name

NID1_MOUSE

NCBI Protein Information

nidogen-1; NID-1; entactin 1; Nidogen precursor (Entactin)

UniProt Comments for Nid1

Nidogen-1: Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell- extracellular matrix interactions. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular region; basement membrane; basal lamina

Molecular Function: laminin-1 binding; collagen binding; protein binding; proteoglycan binding; extracellular matrix binding; laminin binding; calcium ion binding

Biological Process: extracellular matrix organization and biogenesis; cell-matrix adhesion; glomerular basement membrane development; cell adhesion

Research Articles on Nid1

1. Results suggest a non-structural role for nidogen 1 in wound repair.
2. In capillaries, basement membrane formation is severely impaired in the absence of nidogen-1 and -2, showing an irregular, patchy distribution and a dramatically reduced deposition of collagen IV, perlecan, and particularly laminin-411.
3. structure of the interacting domains reveals a six-bladed Tyr-Trp-Thr-Asp (YWTD) beta-propeller domain in nidogen bound to laminin epidermal-growth-factor-like (LE) modules III3-5 in laminin (LE3-5)
4. nidogen-2 is equivalent to nidogen-1 in skin-organotypic coculture, and both can promote the development of a functional basement membrane zone
5. Specific ablation of the nidogen-binding site in the laminin gamma1 chain interferes with kidney and lung development.
6. short arm region of laminin-5 gamma2 chain: structure, mechanism of processing and binding to heparin, nidogen-1, fibulin-1 and fibulin-2
7. Three- to sevenfold increase in nidogen-2 was observed in heart and muscle of mice with nidogen-1 deficiency. Nidogen-2 compensation hypothesis to explain limited phenotype observed following elimination of nidogen-1 gene.
8. Nid1 is essential for late stages of lung development and for maintenance and/or integrity of cardiac tissue
9. nidogen-1 and -2 have roles in development of the ectodermal basement membrane of the limb bud
10. Laminin gamma3 chain, located in murine basement membranes, binds to nidogen
11. expression of the entactin-1 dramatically declined in myogenesis
12. Expression may promote formation of sinusoids after partial hepatectomy.
13. These findings revealed extensive coregulation of nidogen-1 and -2 expression and much more complementary functions of the two nidogens than previously recognized.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-Nid1 antibody

Disease Name	Pubmed Publications
Neoplasm Metastasis Antibodies	>2 publications with Nid1 and Neoplasm Metastasis
Neoplasms Antibodies	>2 publications with Nid1 and Neoplasms
Melanoma Antibodies	>1 publications with Nid1 and Melanoma
Stomach Neoplasms Antibodies	>1 publications with Nid1 and Stomach Neoplasms
Lung Neoplasms Antibodies	>1 publications with Nid1 and Lung Neoplasms
Immune System Diseases Antibodies	>1 publications with Nid1 and Immune System Diseases
Lung Diseases Antibodies	>1 publications with Nid1 and Lung Diseases

Organs/Tissues associated with anti-Nid1 antibody

Organ/Tissue Name	Pubmed Publications
Heart Antibodies	>2 publications with Nid1 and Heart
Brain Antibodies	>2 publications with Nid1 and Brain
Skin Antibodies	>1 publications with Nid1 and Skin
Kidney Antibodies	>1 publications with Nid1 and Kidney
Other Antibodies	>1 publications with Nid1 and Other
Spleen Antibodies	>1 publications with Nid1 and Spleen
Lung Antibodies	>1 publications with Nid1 and Lung