Mouse Anti-CDH1 Monoclonal Antibody – Cat# MBS570170

E-Cadherin / Cadherin-1 (CDH1) Antibody – Mouse Monoclonal

Name: CDH1 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS570170
Price: 605 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS570170

Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis.… More details

$605 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

E-Cadherin / Cadherin-1 (CDH1), Monoclonal Antibody

Full Product Name

Mouse anti E-Cadherin / Cadherin-1

Product Gene Name

anti-CDH1 antibody

Clonality

Monoclonal

Isotype

IgG1

Clone Number

6F9

Host

Mouse

Species Reactivity

Human

Form/Format

Each vial contains 100 ul 1 mg/ml purified antibody in PBS containing 0.09% sodium azide.

Specificity

6F9 recognizes both the 120 kD E-cadherin and its 80 kD trypsin-resistant extracellular part.

Preparation and Storage

The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Molecular Weight

97,456 Da

Chromosome Location

Chromosome: 16; NC_000016.9 (68771195..68869444). Location: 16q22.1

OMIM

137215

3D Structure

ModBase 3D Structure for P12830

Source Note

6F9 is a Mouse monoclonal IgG1 antibody obtained by fusion of P3-X63-Ag 8,653 Mouse myeloma cells with spleen cells from a BABL/c Mouse immunized with affinity purified 80 kD extracellular fragments of E-cadherin derived from tryptic digestion of A-431 Human vulva carcinoma cells.

Product Categories/Family for anti-CDH1 antibody

Cell adhesion; Cancer; Cell adhesion

Applications Tested/Suitable for anti-CDH1 antibody

Immunoblotting, Immunocytochemistry (ICC), Immunohistochemistry (frozen), Western Blotting (WB)

Application Notes for anti-CDH1 antibody

6F9 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. Optimal antibody dilution should be determined by titRation; recommended range is 1:25 - 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:50 - 1:500 for immunoblotting appliCations.

Product References and Citations for anti-CDH1 antibody

Frixen, U. H., Behrens, J., Sachs, M., Eberle, G., Voss, B., Warda, A., Lochner, D., and Birchmeier, W. (1991). E-cadherin-mediated cell-cell adhesion prevents invasiveness of Human carcinoma cells, J Cell Biol 113, 173-85. Schipper, J. H., Frixen, U. H., Behrens, J., Unger, A., Jahnke, K., and Birchmeier, W. (1991). E-cadherin expression in squamous cell carcinomas of head and neck: inverse correlation with tumor dedifferentiation and lymph node metastasis. Cancer Res 51, 6328-6337. Mayer, B., Johnson, J. P., Leitl, F., Jauch, K. W., Heiss, M. M., Schildberg, F. W., Birchmeier, W., and Funke, I. (1993). E-cadherin expression in primary and metastatic gastric cancer: down-regulation correlates with cellular dedifferentiation and glandular disintegRation. Cancer Res 53, 1690-1695. Moll, R., Mitze, M., Frixen, U. H., and Birchmeier, W. (1993). Differential loss of E-cadherin expression in infiltRating ductal and lobular breast carcinomas, Am J Pathol 143, 1731-42. Bohm, M., Totzeck, B., Birchmeier, W., and Wieland, I. (1994). Differences of E-cadherin expression levels and patterns in primary and metastatic Human lung cancer. Clin Exp Metastasis 12, 55-62. Otto, T., Birchmeier, W., Schmidt, U., Hinke, A., Schipper, J., Rubben, H., and Raz, A. (1994). Inverse relation of E-cadherin and autocrine motility factor receptor expression as a prognostic factor in patients with bladder carcinomas. Cancer Res 54, 3120-3123. Zschiesche, W., Schonborn, I., Behrens, J., Herrenknecht, K., Hartveit, F., Lilleng, P., and Birchmeier, W. (1997). Expression of E-cadherin and Catenins in invasive mammary carcinomas. Anticancer Res 17, 561-567. Ghadimi, B. M., Behrens, J., Hoffmann, I., Haensch, W., Birchmeier, W., and Schlag, P. M. (1999). Immunohistological analysis of E-cadherin, alpha-, beta- and gamma-Catenin expression in colorectal cancer: impliCations for cell adhesion and signaling. Eur J Cancer 35, 60-65.

Other Notes

Small volumes of anti-CDH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDH1 purchase

MBS570170 is an antibody targeting CDH1. Also known as Mouse anti E-Cadherin / Cadherin-1. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CDH1. It may not necessarily be applicable to this product.

NCBI GI #

4757960

NCBI GeneID

999

NCBI Accession #

NP_004351.1

UniProt Primary Accession #

P12830

UniProt Secondary Accession #

Q13799; Q14216; Q15855; Q16194; Q4PJ14

UniProt Related Accession #

P12830; Q9UII7; Q9UII8

GenBank Nucleotide #

NM_004360.3

NCBI Official Full Name

cadherin-1 preproprotein

NCBI Official Synonym Full Names

cadherin 1, type 1, E-cadherin (epithelial)

NCBI Official Symbol

CDH1

NCBI Official Synonym Symbols

UVO; CDHE; ECAD; LCAM; Arc-1; CD324

UniProt Protein Name

Cadherin-1

UniProt Synonym Protein Names

CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin

Protein Family

Anaphase-promoting complex

UniProt Gene Name

CDH1

UniProt Synonym Gene Names

CDHE; UVO

UniProt Entry Name

CADH1_HUMAN

NCBI Protein Information

cadherin-1; CAM 120/80; E-Cadherin; uvomorulin; cell-CAM 120/80; OTTHUMP00000174868; epithelial cadherin; cadherin 1, E-cadherin (epithelial); calcium-dependent adhesion protein, epithelial

NCBI Summary for CDH1

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]

UniProt Comments for CDH1

CDH1: a single-pass type I membrane protein, and calcium dependent cell adhesion proteins. It is a ligand for integrin alpha-E/beta-7, and it colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. Has a potent invasive suppressor role.

Protein type: Ubiquitin ligase; Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: apical junction complex; internal side of plasma membrane; cell surface; focal adhesion; basolateral plasma membrane; lateral loop; integral to membrane; extracellular region; catenin complex; trans-Golgi network; actin cytoskeleton; cell-cell adherens junction; apical part of cell; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nerve terminal; cell junction; lateral plasma membrane; endosome

Molecular Function: protein domain specific binding; protein binding; gamma-catenin binding; beta-catenin binding; GTPase activating protein binding; ankyrin binding; calcium ion binding; cell adhesion molecule binding; glycoprotein binding; protein phosphatase binding

Biological Process: response to drug; intercellular junction assembly and maintenance; regulation of immune response; extracellular matrix organization and biogenesis; apoptosis; positive regulation of transcription, DNA-dependent; response to toxin; regulation of caspase activity; trophectodermal cell differentiation; regulation of water loss via skin; extracellular matrix disassembly; cell-cell adhesion; synaptogenesis; sensory perception of sound; pituitary gland development; calcium-dependent cell-cell adhesion; protein metabolic process; positive regulation of transcription factor import into nucleus; negative regulation of cell-cell adhesion; homophilic cell adhesion; protein homooligomerization; neurite development; cell structure disassembly during apoptosis; negative regulation of epithelial cell proliferation

Disease: Gastric Cancer, Hereditary Diffuse; Prostate Cancer; Breast Cancer; Ovarian Cancer; Endometrial Cancer

Research Articles on CDH1

1. Data suggest that SDF-1 down-regulates the E-cadherin/beta-catenin complex expression in HT29 cells by decreasing mRNA synthesis and increasing beta-catenin phosphorylation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CDH1 antibody

Reference Product	PubMed Publications
CTNNB1 antibody	>66 publications with CDH1 and CTNNB1

Pathways associated with anti-CDH1 antibody

Products by Pathway	Pathway Diagram
Adaptive Immunity Signaling Pathway antibodies	Adaptive Immunity Signaling Pathway Diagram
Adherens Junction Pathway antibodies	Adherens Junction Pathway Diagram
Adherens Junction Pathway antibodies	Adherens Junction Pathway Diagram
Adherens Junctions Interactions Pathway antibodies	Adherens Junctions Interactions Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptotic Cleavage Of Cell Adhesion Proteins Pathway antibodies	Apoptotic Cleavage Of Cell Adhesion Proteins Pathway Diagram
Apoptotic Cleavage Of Cellular Proteins Pathway antibodies	Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
Apoptotic Execution Phase Pathway antibodies	Apoptotic Execution Phase Pathway Diagram
Arf6 Trafficking Events Pathway antibodies	Arf6 Trafficking Events Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram

Diseases associated with anti-CDH1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>582 publications with CDH1 and Neoplasms
Carcinoma Antibodies	>281 publications with CDH1 and Carcinoma
Stomach Diseases Antibodies	>191 publications with CDH1 and Stomach Diseases
Stomach Neoplasms Antibodies	>191 publications with CDH1 and Stomach Neoplasms
Adenocarcinoma Antibodies	>167 publications with CDH1 and Adenocarcinoma
Breast Neoplasms Antibodies	>146 publications with CDH1 and Breast Neoplasms
Skin Diseases Antibodies	>123 publications with CDH1 and Skin Diseases
Urogenital Neoplasms Antibodies	>73 publications with CDH1 and Urogenital Neoplasms
Intestinal Diseases Antibodies	>72 publications with CDH1 and Intestinal Diseases
Colorectal Neoplasms Antibodies	>63 publications with CDH1 and Colorectal Neoplasms

Organs/Tissues associated with anti-CDH1 antibody

Organ/Tissue Name	Pubmed Publications
Stomach Antibodies	>202 publications with CDH1 and Stomach
Blood Antibodies	>60 publications with CDH1 and Blood
Liver Antibodies	>44 publications with CDH1 and Liver
Lymph Node Antibodies	>42 publications with CDH1 and Lymph Node
Brain Antibodies	>39 publications with CDH1 and Brain
Lung Antibodies	>37 publications with CDH1 and Lung
Prostate Antibodies	>28 publications with CDH1 and Prostate
Embryonic Tissue Antibodies	>24 publications with CDH1 and Embryonic Tissue
Kidney Antibodies	>24 publications with CDH1 and Kidney
Bladder Antibodies	>16 publications with CDH1 and Bladder