Ep-CAM (EPCAM) Antibody – Rat Monoclonal

Ep-CAM is a 40 kD glycoprotein and can be detected at the basolateral membrane of the majority of epithelial tissues, where it is intriCately linked with the cadherin-Catenin complex and hence the fundamental WNT pathway responsible for intracellular signalling and polarity. This antigen functions as a homotypic calcium-independent cell adhesion molecule. Of particular interest, Ep-CAM appears to be overexpressed by the majority of Human epithelial carcinomas, including colorectal, breast, prostate, head and neck, and hepatic carcinomas. The antigen is being used as a target for immunotherapy of Human carcinomas. Formation of Ep-CAM-mediated adhesions has a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial prolifeRation.

Farr, A., Nelson, A., Truex, J. And Hosier, S. (1991). Epithelial heterogeneity in the murine thymus: a cell surface glycoprotein expressed by subcapsular and medullary epithelium. The Journal of Histochemistry and Cytochemistry 39, 645-53. Lee, M., Sharrow, S., Farr, A., Singer, A. and Udey M. (1994). Expression of the homotypic adhesion molecule E-cadherin by immature murine thymocytes and thymic epithelial cells. Journal of Immunology Mol 152, 5653-59. Borkowski, T., Nelson, A., Farr, A. and Udey, M. (1996). Expression of gp40, the murine homologue of Human epithelial cell adhesion molecule (Ep-Cam), by murine dendritic cells. Eur. J. Immunol. 26, 110-14. Nelson, A., Dunn, R., Peach, R., Aruffo, A. and Farr, A. (1996). The murine homolog of Human Ep-CAM, a homotypic adhesion molecule, is expressed by thymocytes and thymic epithelial cells. Eur. J. Immunol. 26, 401-408. Went, P., Vasei, M., Bubendorf, L., Terracciano, L., Tornillo, L., Riede, U., Kononen, J., Simon, R., Sauter, G. And Baeuerle, P.A. (2006). Frequent high-level expression of the immunotherapeutic target Ep-CAM in colon, stomach, prostate and lung cancers bladder. British Journal of Cancer 94, 128-135. Maaser, K. and Borlak, J. (2008). A genom-wide expression analysis identifies a network of EpCAM-induced cel cycle regulators. British Journal of Cancer 99, 1635-43

Small volumes of anti-EPCAM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS570187 is an antibody targeting EPCAM. Also known as Rat anti Ep-CAM. Reactive in Mouse. Host: Rat.