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Actin Alpha-Skeletal (ACTA1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS570258

Among the six actin isoforms described in mammals, two are found in virtually all cells (beta- and gamma-cytoplasmic), two are detected in smooth muscle cells (alpha- and gamma-smooth muscle) and two are present in striated muscles, one predominantly in… More details

$605 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
actin alpha-skeletal (ACTA1), Monoclonal Antibody
Full Product Name
Mouse anti actin alpha-skeletal
Product Gene Name
anti-ACTA1 antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3B3
Host
Mouse
Species Reactivity
Caprine, Human, Rabbit, Rat, Swine.
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Specificity
Clone 3B3 is an antibody highly specific for alpha-skeletal actin, and does not cross react with other actin isoforms.
Preparation and Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Chromosome Location
Chromosome: 1; NC_000001.10 (229566992..229569858, complement). Location: 1q42.13
OMIM
102610
3D Structure
ModBase 3D Structure for P68133
Source Note
3B3 is a Mouse monoclonal IgG1 antibody derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of alpha-skeletal actin with an acetylated N-terminus (Ac-DEDETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.
Reactivity Note
The epitope recognized by alpha-SM1 is highly conserved. The antibody therefore cross-reacts with many species including protochordates, lower craniates and mammals.
Product Type
Primary Antibody

Product Categories/Family for anti-ACTA1 antibody

Cytoskeletal; Cardiovascular; Cell differentiation; Cytoskeleton; Stem cell research
Applications Tested/Suitable for anti-ACTA1 antibody
ELISA, Immunoblotting, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blotting.
Application Notes for anti-ACTA1 antibody
3B3 is useful for immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting and ELISA. Optimal antibody dilutions should be determined by titRation; recommended range is 1:100 - 1:1000 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:1000 - 1:5000 for immunoblotting appliCations.

Testing Data of anti-ACTA1 antibody

Paraffin sections of human striated skeletal muscle (above) and striated muscle (below) from human tongue immunostained with 3B3 (1:250)
Mouse Anti-ACTA1 Monoclonal Antibody – Cat# MBS570258

Testing Data of anti-ACTA1 antibody

Mouse Anti-ACTA1 Monoclonal Antibody, Testing Data – Cat# MBS570258

Product References and Citations for anti-ACTA1 antibody

Vandekerckhove, J. and Weber, K. (1978). At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4), 783-802.Vandekerckhove, J., Bugaisky, G. and Buckingham, M. (1986). Simultaneous expression of skeletal muscle and heart actin proteins in various striated muscle tissues and cells. J Biol Chem. 261(4),1838-43.Suurmeijer, A.J., Clément, S., Francesconi, A., Bocchi, L., Angelini, A., Van Veldhuisen, D.J., Spagnoli, L.G., Gabbiani, G. and Orlandi, A. (2003). Alpha-actin isoform distribution in normal and failing Human heart: a morphological, morphometric, and biochemical study. J Pathol. 199(3), 387-97.Chaponnier, C. and Gabbiani, G. (2004). Pathological situations characterized by altered actin isoform expression. J Pathol. 204(4), 386-95.Driesen, R.B., Verheyen F.K., Debie, W., Blaauw, E., Babiker, F.A., Cornelussen, R.N., Ausma, J., Lenders, M.H., Borgers, M., Chaponnier, C. and Ramaekers, F.C. (2009). Re-expression of alpha skeletal actin as a marker for dedifferentiation in cardiac pathologies. J Cell Mol Med 13(5), 896-908.

Other Notes

Small volumes of anti-ACTA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACTA1 purchase

MBS570258 is an antibody targeting ACTA1. Also known as Mouse anti actin alpha-skeletal. Reactive in Caprine, Human, Rabbit, Rat, Swine.. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTA1. It may not necessarily be applicable to this product.

NCBI GI #
4501881
NCBI GeneID
58
NCBI Accession #
NP_001091.1
UniProt Primary Accession #
P68133
UniProt Secondary Accession #
P02568; P99020; Q5T8M9
UniProt Related Accession #
P68133
GenBank Nucleotide #
NM_001100.3
NCBI Official Full Name
actin, alpha skeletal muscle
NCBI Official Synonym Full Names
actin, alpha 1, skeletal muscle
NCBI Official Symbol
ACTA1
NCBI Official Synonym Symbols
ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
UniProt Protein Name
Actin, alpha skeletal muscle
UniProt Synonym Protein Names
Alpha-actin-1
Protein Family
Actin
UniProt Gene Name
ACTA1
UniProt Synonym Gene Names
ACTA
UniProt Entry Name
ACTS_HUMAN
NCBI Protein Information
actin, alpha skeletal muscle; nemaline myopathy type 3

NCBI Summary for ACTA1

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]
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UniProt Comments for ACTA1

ACTA1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells. Belongs to the actin family.

Protein type: Cell development/differentiation; Contractile; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q42.13

Cellular Component: extracellular space; sarcomere; stress fiber; actin filament; cytosol; striated muscle thin filament; actin cytoskeleton

Molecular Function: protein binding; myosin binding; structural constituent of cytoskeleton; ADP binding; ATP binding

Biological Process: skeletal muscle fiber adaptation; muscle contraction; response to steroid hormone stimulus; response to mechanical stimulus; muscle thin filament assembly; response to lithium ion; response to extracellular stimulus; skeletal muscle fiber development; cell growth; muscle filament sliding

Disease: Nemaline Myopathy 3; Myopathy, Congenital, With Fiber-type Disproportion
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Research Articles on ACTA1

1. These findings suggest that the direct contact of Dengue virus 2 E protein with 43 kDa actin protein may have a crucial function in Dengue virus 2 infection of ECV304 cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ACTA1 antibody

 Reference Product  PubMed Publications
 TPM3 antibody  >26 publications with ACTA1 and TPM3
 ACTC1 antibody  >1 publications with ACTA1 and ACTC1

Pathways associated with anti-ACTA1 antibody

 Products by Pathway  Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Hypothetical Network For Drug Addiction Pathway antibodies  Hypothetical Network For Drug Addiction Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 RhoA Signaling Pathway antibodies  RhoA Signaling Pathway Diagram
 Signaling Events Mediated By Focal Adhesion Kinase Pathway antibodies  Signaling Events Mediated By Focal Adhesion Kinase Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram

Diseases associated with anti-ACTA1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >78 publications with ACTA1 and Nervous System Diseases
 MYOPATHY, CONGENITAL, WITH FIBER-TYPE DISPROPORTION Antibodies  >60 publications with ACTA1 and MYOPATHY, CONGENITAL, WITH FIBER-TYPE DISPROPORTION
 Nemaline myopathy 3 Antibodies  >16 publications with ACTA1 and Nemaline myopathy 3
 Hypertrophy Antibodies  >14 publications with ACTA1 and Hypertrophy
 Heart Diseases Antibodies  >14 publications with ACTA1 and Heart Diseases
 Cardiovascular Diseases Antibodies  >12 publications with ACTA1 and Cardiovascular Diseases
 Atrophy Antibodies  >7 publications with ACTA1 and Atrophy
 Fibrosis Antibodies  >6 publications with ACTA1 and Fibrosis
 Fetal Death Antibodies  >3 publications with ACTA1 and Fetal Death
 Hepatomegaly Antibodies  >2 publications with ACTA1 and Hepatomegaly

Organs/Tissues associated with anti-ACTA1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >109 publications with ACTA1 and Muscle
 Heart Antibodies  >22 publications with ACTA1 and Heart
 Brain Antibodies  >9 publications with ACTA1 and Brain
 Embryonic Tissue Antibodies  >6 publications with ACTA1 and Embryonic Tissue
 Bone Antibodies  >4 publications with ACTA1 and Bone
 Nerve Antibodies  >3 publications with ACTA1 and Nerve
 Lung Antibodies  >3 publications with ACTA1 and Lung
 Eye Antibodies  >2 publications with ACTA1 and Eye
 Kidney Antibodies  >2 publications with ACTA1 and Kidney
 Liver Antibodies  >2 publications with ACTA1 and Liver
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