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VCAM-1 (VCAM1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS570289

Vascular cell adhesion molecule-1 (VCAM-1) is induced on Human umbilical vein endothelium (HUVE) by recombinant Human tumor necrosis factor - alpha (rh TNF-alpha), rh interleukin-1 (IL-1) and lipopolysaccharide (LPS) and is involved in adherence of… More details

$605 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
VCAM-1 (Vcam1), Monoclonal Antibody
Full Product Name
Mouse anti VCAM-1
Product Gene Name
anti-Vcam1 antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4B9
Host
Mouse (Balb/c)
Species Reactivity
Human
Form/Format
Each vial contains 100 ul of 1 mg/ml purified antibody in PBS containing 0.09% sodium azide.
Specificity
The antibody binds exclusively to the cytokine-induced endothelial cell adhesion protein, vascular cell adhesion molecule-1 (VCAM-1).
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Molecular Weight
81,246 Da
Chromosome Location
Chromosome: 2; NC_005101.3 (237158201..237177689, complement). Location: 2q41
3D Structure
ModBase 3D Structure for P29534
Source Note
4B9 is a Mouse IgG1 monoclonal antibody geneRated by immunization of Balb/c mice with Human umbilical vein endothelium (HUVE) that were non-enzymatically harvested after stimulation for 24h with recombinant Human (rh) TNF-alpha (10 ng/ml). Splenocytes were fused with the NS-1 nonsecretory murine myeloma line.

Product Categories/Family for anti-Vcam1 antibody

Tumour marker; Cancer; Immunology
Applications Tested/Suitable for anti-Vcam1 antibody
Flow Cytometry (FC), Immunohistochemistry (IHC) (frozen), Immunoprecipitation (IP)
Application Notes for anti-Vcam1 antibody
4B9 is suitable for immunoblotting, immunoprecipitation, immunocytochemistry and immuno-histochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.

Product References and Citations for anti-Vcam1 antibody

Carlos. T., Schwartz. B., Kovach. N., Yee. E., Rosa. M., Osborn. L., Chi-Rosso. G., Newman. B., Lobb. R., et al. (1990). Vascular cell adhesion molecule-1 mediates lymphocyte adherence to cytokine-activated cultured Human endothelial cells Blood. 76(5) 965-70. Ockenhouse, C.F., Tegoshi, T., Maeno, Y., Benjamin, C., Ho, M., Kan, K.E., Thway, Y., Win, K., Aikawa, M. and Lobb, R.R. (1992). Human vascular endothelial cell adhesion receptors for Plasmodium falciparum-infected erythrocytes: roles for endothelial leukocyte adhesion molecule 1 and vascular cell adhesion molecule 1. J Exp Med. 176, 1183-9. Bacchi, C.E., Marsh, C.L., Perkins, J.D., Carithers, R.L. Jr, McVicar, J.P., Hudkins, K.L., Benjamin, C.D., Harlan, J.M., Lobb, R. and Alpers, C.E. (1993). Expression of vascular cell adhesion molecule (VCAM-1) in liver and pancreas allograft rejection. Am J Pathol. 142, 579-91. Pinola, M., Saksela, E., Tiisala, S. and Renkonen, R. (1994). Human NK cells expressing alpha 4 beta 1/beta 7 adhere to VCAM-1 without preactivation. Scand J Immunol. 39, 131-6. Chuluyan, H.E., Osborn, L., Lobb, R. and Issekutz AC. (1995). Domains 1 and 4 of vascular cell adhesion molecule-1 (CD106) both support very late activation antigen-4 (CD49d/CD29)-dependent monocyte trans-endothelial migRation. J Immunol. 155, 3135-4.

Other Notes

Small volumes of anti-Vcam1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Vcam1 purchase

MBS570289 is an antibody targeting Vcam1. Also known as Mouse anti VCAM-1. Reactive in Human. Host: Mouse (Balb/c).

Additional Details

NCBI/Uniprot data below describe general gene information for Vcam1. It may not necessarily be applicable to this product.

NCBI GI #
6981700
NCBI GeneID
25361
NCBI Accession #
NP_037021.1
UniProt Primary Accession #
P29534
UniProt Secondary Accession #
Q5FVS3; Q63669
UniProt Related Accession #
P29534
GenBank Nucleotide #
NM_012889.1
NCBI Official Full Name
vascular cell adhesion protein 1
NCBI Official Synonym Full Names
vascular cell adhesion molecule 1
NCBI Official Symbol
Vcam1
NCBI Official Synonym Symbols
VCAM1B
UniProt Protein Name
Vascular cell adhesion protein 1
Protein Family
Vascular cell adhesion protein
UniProt Gene Name
Vcam1
UniProt Synonym Gene Names
Vcam-1; V-CAM 1; VCAM-1
UniProt Entry Name
VCAM1_RAT
NCBI Protein Information
vascular cell adhesion protein 1; VCAM-1; V-CAM 1

NCBI Summary for Vcam1

binds integrin very late antigen (VLA4); plays a role in cell adhesion [RGD, Feb 2006]

UniProt Comments for Vcam1

VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral

Cellular Component: Golgi apparatus; extracellular space; cell surface; microvillus; endoplasmic reticulum; apical part of cell; early endosome; integral to membrane; podosome; sarcolemma; filopodium; external side of plasma membrane

Molecular Function: integrin binding; amine oxidase activity; cell adhesion molecule binding

Biological Process: response to nicotine; chronic inflammatory response; viral reproduction; cell-matrix adhesion; heart development; response to lipopolysaccharide; heterophilic cell adhesion; leukocyte adhesion; response to ethanol; response to zinc ion; membrane to membrane docking; response to hypoxia; positive regulation of T cell proliferation; response to ionizing radiation; amine metabolic process; leukocyte tethering or rolling; cell adhesion; acute inflammatory response; response to nutrient; aging
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Research Articles on Vcam1

1. These findings suggested that glioma-induced change in VCAM-1 expression of bone marrow stem cells may play an important role in their tropism towards glioma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Vcam1 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway antibodies  Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway Diagram
 Integrin Cell Surface Interactions Pathway antibodies  Integrin Cell Surface Interactions Pathway Diagram

Diseases associated with anti-Vcam1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >58 publications with Vcam1 and Inflammation
 Cardiovascular Diseases Antibodies  >43 publications with Vcam1 and Cardiovascular Diseases
 Necrosis Antibodies  >41 publications with Vcam1 and Necrosis
 Neoplasms Antibodies  >34 publications with Vcam1 and Neoplasms
 Atherosclerosis Antibodies  >30 publications with Vcam1 and Atherosclerosis
 Kidney Diseases Antibodies  >10 publications with Vcam1 and Kidney Diseases
 Hypertension Antibodies  >10 publications with Vcam1 and Hypertension
 Heart Diseases Antibodies  >9 publications with Vcam1 and Heart Diseases
 Myocardial Ischemia Antibodies  >7 publications with Vcam1 and Myocardial Ischemia
 Myocardial Infarction Antibodies  >6 publications with Vcam1 and Myocardial Infarction

Organs/Tissues associated with anti-Vcam1 antibody

 Organ/Tissue Name  Pubmed Publications
 Artery Antibodies  >22 publications with Vcam1 and Artery
 Kidney Antibodies  >19 publications with Vcam1 and Kidney
 Embryonic Tissue Antibodies  >19 publications with Vcam1 and Embryonic Tissue
 Brain Antibodies  >14 publications with Vcam1 and Brain
 Lung Antibodies  >7 publications with Vcam1 and Lung
 Adipose Tissue Antibodies  >5 publications with Vcam1 and Adipose Tissue
 Spleen Antibodies  >4 publications with Vcam1 and Spleen
 Eye Antibodies  >3 publications with Vcam1 and Eye
 Pancreas Antibodies  >3 publications with Vcam1 and Pancreas
 Prostate Antibodies  >2 publications with Vcam1 and Prostate
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