MT1 Antibody – Mouse Monoclonal

Monoclonal

IgG1

MT1

Mouse

Human

Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Clone MT1 produces Mouse IgG1 immunoglobulins reactive with a 95-115 kD highly sialated glycoprotein. This antibody is used to identify B cell lines and myeloma cells. It may be used in the chronic lymphocytic leukemias (CLL), as an alternative to CD5. The antigen detected by MT1 is the only marker expressed on neoplasms of the very early precursor cells. In immunohistochemistry it reacts with T cells, macrophages, myeloid cells and B cells (weak). It is used for the typing of lymphomas in paraffin sections. CD43 may function as an adhesion molecule via interaction with CD54 although this has not been definitely established. It may also inhibit leucocyte interactions with other cells. The antigen is involved in the activation of T-cells, B cells, NK cells and monocytes. The membrane proximal portion of the cytoplasmic domain mediates an association with the cytoskeleton.

Store at 4 degree C, or in small aliquots at -20 degree C.

MT1 is a monoclonal antibody derived by fusion of X63 Mouse myeloma cells with spleen cells from of Balb/c mice, intraperitoneally immunized with Human monocytes isolated by centrifugal elutriation. Immunizations were carried out at least twice at 2-week intervals with: A) cell suspensions of a lymph node involved by Hodgkin's disease; B) cell suspensions of a lymph node involved by Bchronic lymphatic leukemia; C) cells of Hodgkin's cell line DEV, respectively. No adjuvants were used (3).

CD markers; Immunology