Mouse Anti-Tenascin C Monoclonal Antibody – Cat# MBS570322

Tenascin C Antibody – Mouse Monoclonal

Name: Tenascin C Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS570322
Price: 605 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS570322

Tenascin is a high molecular weight, multifunctional, extracellular matrix glycoprotein expressed in association with mesenchymal-epithelial interactions during development. It has been described under a variety of names, i.e. cytotactin, hexabrachion… More details

$605 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Tenascin C, Monoclonal Antibody

Full Product Name

Mouse anti Tenascin C

Clonality

Monoclonal

Isotype

IgG1

Clone Number

T2H5

Host

Mouse

Species Reactivity

Human

Form/Format

Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Specificity

In immunoblotting assays on an extract of a human osteosarcoma cell line (U2OS) T2H5 recognizes a 265 kDa protein. Tenascin C contains an exstensive number of potential glycosylation sites, which may explain its migration at a higher molecular weight than predicted. Human tonsilar tissue can be used as a positive control in immunocytochemistry and immunohistochemistry.

Preparation and Storage

Store at 4 degree C, or in small aliquots at -20 degree C.

Molecular Weight

9,764 Da

Source Note

T2H5 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a BALB/c mouse immunized with full length native tenascin protein purified from a homogenate of a human mammary tumour specimen.

Product Categories/Family for anti-Tenascin C antibody

Neurosciences; Oncology

Applications Tested/Suitable for anti-Tenascin C antibody

Immunohistochemistry on frozen and paraffin sections, Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB)

Application Notes for anti-Tenascin C antibody

T2H5 is suitable for immunoblotting, immunocytochemistry, and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Product References and Citations for anti-Tenascin C antibody

1) Tokes AM, Hortovanyi E, Csordas G, Kulka J, Mozes G, Hatalyak A, Kadar A: Immunohistochemical localisation of tenascin in invasive ductal carcinoma of the breast. Anticancer Res. 1999;19:175-179.

2) Faustino AM, van Garderen E, Schalken JA, Nederbragt H: Tenascin expression in normal, hyperplastic, dysplastic and neoplastic canine mammary tissues. J Comp Pathol. 2002;126:1-8.

3) Gulubova M: Immunohistochemical localization of collagen type III and type IV, laminin, tenascin and alpha-smooth muscle actin (alphaSMA) in the human liver in peliosis. Pathol Res Pract. 2002;198:803-812.

4) Ioachim E, Charchanti A, Briasoulis E, Karavasilis V, Tsanou H, Arvanitis DL, Agnantis NJ, Pavlidis N: Immunohistochemical expression of extracellular matrix components tenascin, fibronectin, collagen type IV and laminin in breast cancer: their prognostic value and role in tumour invasion and progression. Eur J Cancer. 2002;38:2362-2370.

5) Singh SR, Billington CK, Sayers I, Hall IP. Can lineage-specific markers be identified to characterize mesenchyme-derived cell populations in the human airways? Am J Physiol Lung Cell Mol Physiol. 2010;299:L169-183.

Other Notes

Small volumes of anti-Tenascin C antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Tenascin C purchase

MBS570322 is an antibody targeting Tenascin C. Also known as Mouse anti Tenascin C. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Tenascin C. It may not necessarily be applicable to this product.

NCBI GI #

1842130

NCBI Accession #

AAB47574.1

NCBI Official Full Name

tenascin-C, partial

UniProt Protein Name

Tenascin-C

UniProt Synonym Protein Names

Tenascin-C

Protein Family

Tenascin

UniProt Entry Name

Q99857_HUMAN

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.