Mouse Anti-LTF Antibody – Cat# MBS570366

Lactoferrin (LTF) Antibody – Mouse

Name: LTF Antibody; Mouse; Human-Reactive; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS570366
Price: 795 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS570366

Lactoferrin (LF) is an iron-binding protein with bactericidal and bacteriostatic activity, which is stored within the secondary granules of granulocytes. LF expression is restricted to the post-mitotic maturation compartment of the granulocytic lineage,… More details

$795 / 2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Lactoferrin (LTF), Antibody

Full Product Name

Mouse anti Lactoferrin, conjugated to PE

Product Gene Name

anti-LTF antibody

Isotype

IgG1

Clone Number

4C5

Host

Mouse

Species Reactivity

Human

Form/Format

Purified by Chromatography, Storage buffer: PBS pH 7.2, 1% BSA, 0.05% NaN3

Specificity

The LF mAb (clone 4C5) recognizes Lactoferrin stored within secondary granules of postmitotic granulocyte-committed cells.

The sensitivity of 4C5 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells). In practice, 50 ul of leukocytes containing 10^7
cells/ml are stained with 20 ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.

Preparation and Storage

Monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody. For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8 degree C (DO NOT FREEZE!) and protected from prolonged exposure to light. Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended.

Molecular Weight

73,161 Da

OMIM

gene 245480

CE Mark

Conjugation

Applications Tested/Suitable for anti-LTF antibody

Flow Cytometry (FC/FACS), Indirect Immunofluorescence (IF)

Application Notes for anti-LTF antibody

Permeabilization and Staining Procedure
- In combination with our Permeabilization Kit FIX&PERM intracellular Lactoferrin can be easily stained in cell suspensions.
- For each sample to be analyzed add 50 ul of whole blood, bone marrow or mononuclear cell suspension in a 5ml tube
- Add 100 ul of Reagent A (Fixation Medium, stored and used at room temperature)
- Incubate for 15 minutes at room temperature
- Add 5ml phosphate buffered saline and centrifuge cells for 5 minutes at 300 g
- Remove supernatant and add to cell pellet 100 ul Reagent B (Permeabilization Medium) and 20 ul of the Lactoferrin monoclonal antibody conjugate
- Vortex at low speed for 1-2 seconds
- Incubate for 15 minutes at room temperature
- Wash cells with phosphate buffered saline as described above
- Remove supernatant and resuspend cells in sheath fluid for immediate analysis or resuspend cells in 0.5 ml 1.0 % formaldehyde and store them at 2-8 degree C in the dark. Analyze fixed
cells within 24 hours.

Conventional Staining for Microscopic Evaluations
LF-Antibody 4C5 can also be used to demonstrate lactoferrin molecules by conventional immunofluorescence or immunoenzyme staining techniques on cell smears, cytospin
preparations or tissue sections. Acetone or paraformaldehyde are suitable fixatives for these purposes.

Product References and Citations for anti-LTF antibody

Braylan, R. C., Orfao, A., Borowitz, M. J. & Davis, B. H. (2001) Cytometry 46, 23-7.
Catovsky, D., Matutes, E., Buccheri, V., Shetty, V., Hanslip, J., Yoshida, N. & Morilla, R. (1991) Ann Hematol 62, 16-21.
Cowland, J. B. & Borregaard, N. (1999) J Leukoc Biol 66, 989-95
Cramer, E., Pryzwansky, K. B., Villeval, J. L., Testa, U. & Breton-Gorius, J. (1985) Blood 65, 423-32.
Groeneveld, K., te Marvelde, J. G., van den Beemd, M. WHooijkaas, H. & van Dongen, J. J. (1996) Leukemia 10, 1383-9.
Gullberg, U., Andersson, E., Garwicz, D., Lindmark, A. & Olsson, I. (1997) Eur J Haematol 58, 137-53.
He, J. & Furmanski, P. (1995) Nature 373, 721-4
Knapp, W., Majdic, O. & Strobl, H. (1993) Recent Results Cancer Res 131, 31-40.
Konikova, E., Glasova, M., Kusenda, J. & Babusikova, O. (1998) Neoplasma 45, 282-91.
Oehler, L., Majdic, O., Pickl, W. F., Stockl, J., Riedl, E., Drach, J., Rappersberger, K., Geissler, K. & Knapp, W. (1998) J Exp Med 187, 1019-28.
Paietta, E. (2003) Best Pract Res Clin Haematol 16, 671-83.
Rado, T. A., Bollekens, J., St Laurent, G., Parker, L. & Benz, E. J., Jr. (1984) Blood 64, 1103-9.
Rado, T. A., Wei, X. P. & Benz, E. J., Jr. (1987) Blood 70, 989-93.
Srivastava, C. H., Rado, T. A., Bauerle, D. & Broxmeyer, H. E. (1991) J Immunol 146, 1014-9.
Strobl, H. & Knapp, W. (2004) J Biol Regul Homeost Agents 18, 335-9.
Teng, C. T., Gladwell, W., Beard, C., Walmer, D., Teng, C. S. & Brenner, R. (2002) Mol Hum Reprod 8, 58-67.

Other Notes

Small volumes of anti-LTF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LTF purchase

MBS570366 is an antibody targeting LTF. Also known as Mouse anti Lactoferrin, conjugated to PE. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for LTF. It may not necessarily be applicable to this product.

NCBI GI #

2104522

NCBI GeneID

4057

NCBI Accession #

AAB57795.1

UniProt Secondary Accession #

O00756; Q16780; Q16785; Q16786; Q16789; Q5DSM0; Q8IU92; A8K9U8; B2MV13; B7Z4X2; E7EQH5

UniProt Related Accession #

P02788

NCBI Official Full Name

lactoferrin, partial

NCBI Official Synonym Full Names

lactotransferrin

NCBI Official Symbol

LTF

NCBI Official Synonym Symbols

LF; HLF2; GIG12; HEL110

UniProt Protein Name

Lactotransferrin

UniProt Synonym Protein Names

Growth-inhibiting protein 12; TalalactoferrinCleaved into the following 5 chains:Lactoferricin-H; Lfcin-H; Kaliocin-1; Lactoferroxin-A; Lactoferroxin-B; Lactoferroxin-C

Protein Family

L-shaped tail fiber protein

UniProt Gene Name

LTF

UniProt Synonym Gene Names

GIG12; LF; Lactoferrin; Lfcin-H

UniProt Entry Name

TRFL_HUMAN

NCBI Protein Information

lactotransferrin; kaliocin-1; lactoferricin; lactoferroxin; talalactoferrin; neutrophil lactoferrin; growth-inhibiting protein 12; epididymis luminal protein 110

NCBI Summary for LTF

This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

UniProt Comments for LTF

LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Secreted; EC 3.4.21.-; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: extracellular space; specific granule; cytoplasm; extracellular region; nucleus; secretory granule

Molecular Function: heparin binding; protein serine/threonine kinase activator activity; protein binding; DNA binding; ferric iron binding; iron ion binding; serine-type endopeptidase activity

Biological Process: negative regulation of lipopolysaccharide-mediated signaling pathway; interaction with host; ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; positive regulation of toll-like receptor 4 signaling pathway; antifungal humoral response; proteolysis; iron ion transport; humoral immune response; regulation of cytokine production; activation of NF-kappaB transcription factor; retinal homeostasis; positive regulation of osteoblast differentiation; negative regulation of viral genome replication; innate immune response in mucosa; response to host immune response; positive regulation of osteoblast proliferation; antibacterial humoral response; regulation of tumor necrosis factor production; iron assimilation by chelation and transport; negative regulation of viral reproduction; negative regulation of ATPase activity; negative regulation of apoptosis

Research Articles on LTF

1. The LTF 140A/G (exon 2, Lys/Arg) polymorphism was not associated with the susceptibility to or severity of dental caries in the Czech population.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-LTF antibody

Products by Pathway	Pathway Diagram
Amyloids Pathway antibodies	Amyloids Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Latent Infection Of Homo Sapiens With Mycobacterium Tuberculosis Pathway antibodies	Latent Infection Of Homo Sapiens With Mycobacterium Tuberculosis Pathway Diagram
Mtb Iron Assimilation By Chelation Pathway antibodies	Mtb Iron Assimilation By Chelation Pathway Diagram
Phagosomal Maturation (early Endosomal Stage) Pathway antibodies	Phagosomal Maturation (early Endosomal Stage) Pathway Diagram
Response Of Mtb To Phagocytosis Pathway antibodies	Response Of Mtb To Phagocytosis Pathway Diagram

Diseases associated with anti-LTF antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>56 publications with LTF and Nervous System Diseases
Immune System Diseases Antibodies	>38 publications with LTF and Immune System Diseases
Inflammation Antibodies	>27 publications with LTF and Inflammation
Cardiovascular Diseases Antibodies	>19 publications with LTF and Cardiovascular Diseases
Vascular Diseases Antibodies	>16 publications with LTF and Vascular Diseases
Lung Diseases Antibodies	>15 publications with LTF and Lung Diseases
Hypertension Antibodies	>8 publications with LTF and Hypertension
Necrosis Antibodies	>7 publications with LTF and Necrosis
Liver Diseases Antibodies	>7 publications with LTF and Liver Diseases
Breast Neoplasms Antibodies	>7 publications with LTF and Breast Neoplasms

Organs/Tissues associated with anti-LTF antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>125 publications with LTF and Blood
Brain Antibodies	>85 publications with LTF and Brain
Muscle Antibodies	>52 publications with LTF and Muscle
Lung Antibodies	>22 publications with LTF and Lung
Uterus Antibodies	>17 publications with LTF and Uterus
Liver Antibodies	>15 publications with LTF and Liver
Skin Antibodies	>15 publications with LTF and Skin
Eye Antibodies	>14 publications with LTF and Eye
Bone Antibodies	>13 publications with LTF and Bone
Intestine Antibodies	>10 publications with LTF and Intestine