CD45 Antibody – Mouse

The CD45 molecule is typically expressed at high levels on all hematopoietic cells. CD45 is a major component of the glycocalix of these cells and can be expressed in different isoforms. Antibody VIT200 recognizes a pan CD45 epitope, which is expressed on all hematopoietic cells. CD14 is a GPI-anchored molecule expressed by virtually all human monocytes and macrophages and - to a lesser degree - granulocytes. CD14 together with Toll-like receptor 4 and MD-2 forms the LPS-receptor complex that recognizes and signals the presence of LPS. While CD14 has no signaling structure its main role seems to be the binding of LPS.

The VIT200/MEM18 COMBI-REAGENT-Gate Control permits the identification and enumeration of human leukocytes using flow cytometry.

Results must be put within the context of other tests as well as the clinical history of the patient by a certified professional before final interpretation. Analysis performed with this antibody should be paralleled by positive and negative controls.

Battifora, H. & Trowbridge, I. S. (1983) Cancer 51, 816-21.
Beutler, B. (2002) Curr Top Microbiol Immunol 270, 109-20.
Brocklebank, A. M. & Sparrow, R. L. (2001) Cytometry 46, 254-61.
Cobbold, S., Hale, G. & Waldmann, H. (1987) Leucocyte Typing III. p788-803 (Oxford University Press, Oxford-New York-Tokyo).
Dalchau, R., Kirkley, J. & Fabre, J. W. (1980) Eur J Immunol 10, 737-44.
Goyert, S. M. (1989) Leucocyte Typing IV. p789-793 (Oxford University Press, Oxford-New York-Tokyo).
Goyert, S. M., Ferrero, E., Rettig, W. J., Yenamandra, A. K., Obata, F. & Le Beau, M. M. (1988) Science 239, 497-500.
Goyert, S. M., Ferrero, E. M., Seremetis, S. V., Winchester, R. J., Silver, J. & Mattison, A. C. (1986) J Immunol 137, 3909-14.
Jing, S., Ralph, S., Head, M. T. A., Chain, A. & Trowbridge, I. (1987) Structural studies of the transferrin receptor and T 200 glycoprotein (CD45). Leucocyte Typing III. p899-905 (Oxford University Press, Oxford-New York-Tokyo).
Knapp, W. (1982) Blut 45, 301-8.
Knapp, W. (1989) Leucocyte Typing IV. p747-780 (Oxford University Press, Oxford-New York-Tokyo).
Means, T. K., Lien, E., Yoshimura, A., Wang, S., Golenbock, D. T. & Fenton, M. J. (1999) J Immunol 163, 6748-55.
Nicholson, J. K., Hubbard, M. & Jones, B. M. (1996) Cytometry 26, 16-21.
Sugita, K., Majdic, O., Stockinger, H., Holter, W., Burger, R. & Knapp, W. (1987) Transplantation 43, 570-4.
Sun, T., Sangaline, R., Ryder, J., Gibbens, K., Rollo, C., Stewart, S. & Rajagopalan, C. (1997) Am J Clin Pathol 108, 152-7.
Tapping, R. I., Akashi, S., Miyake, K., Godowski, P. J. & Tobias, P. S. (2000) J Immunol 165, 5780-7.
Thomas, M. L. (1989) Annu Rev Immunol 7, 339-69.
Ugolini, V., Nunez, G., Smith, R. G., Stastny, P. & Capra, J. D. (1980) Proc Natl Acad Sci U S A 77, 6764-8.
Yoshimura, A., Lien, E., Ingalls, R. R., Tuomanen, E., Dziarski, R. & Golenbock, D. (1999) J Immunol 163, 1-5.

Small volumes of anti-CD45 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS570370 is an antibody targeting CD45. Also known as COMBI IC Reagent: Mouse anti CD45 (FITC) and Mouse anti CD14 (PE). Reactive in Human. Host: Mouse.