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Flow Cytometry Lysing Solution Reagent

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS570406

For Wash- or No-Wash Lysing Procedures with Whole Blood or Marrow SamplesNM-LYSE is a premixed, ready to use lysing solution fomulated for lysing erythrocytes following monoclonal antibody staining of whole blood. Treatment with this reagent simultaneously… More details

$350 / 30 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Flow Cytometry Lysing Solution, Reagent
Full Product Name
NM LYSE: Flow Cytometry Lysing Solution
Specificity
NM-LYSE Reagent is designed for use with all commercially available flow cytometers. Alignment and compensation should be
performed according to manufacturer´s instructions

The quality of each NM-LYSE Lot is determined by lysing red blood cells of well defined blood samples from representative
donors and subsequent comparison of forward and side scatter characteristics of obtained leukocytes.
Preparation and Storage
Reagent should be stored and used at room temperature.
Stability of the reagent: Please refer to the expiry date printed onto
the vial. The use of the reagent after the expiration date is not
recommended. Do not use reagent if a precipitate should form or
discoloration occurs.
If unexpected results are obtained which cannot be attributed to
differences in laboratory procedures, please contact us
CE Mark
CE
Applications Tested/Suitable for Flow Cytometry Lysing Solution reagent
Flow Cytometry (FC/FACS)
No-Wash Staining and Lysing Procedure
- For each sample add 50 ul of EDTA anti-coagulated blood to a 3-5 ml tube
- Add 20 ul of the appropriate monoclonal antibody conjugate
- Incubate the tube for 15 minutes at 4 degree C or at room temperature in the dark
- Add 100 ul NM-LYSE to each tube and incubate for 10 minutes at room temperature
- Add 1 ml of destilled water and vortex, incubate for 5-10 minutes at room temperature
- Analyze immediately or store samples at 2-8 degree C in the dark and analyze within 24 hours

Wash Staining and Lysing Procedure
- For each sample add 50 ul of EDTA anti-coagulated blood to a 3-5 ml tube
- Add 20 ul of the appropriate monoclonal antibody conjugate
- Incubate the tube for 15 minutes at 4 degree C or at room temperature in the dark
- Add 100 ul NM-LYSE to each tube and incubate for 10 minutes at room temperature
- Add 3-4 ml of destilled water and vortex, incubate for 5-10 minutes at room temperature
- Centrifuge tube for 5 minutes at 300 g
- Aspirate supernatant and resuspend pellet in 0.3 ml of sheath fluid
- Analyze immediately or store samples at 2-8 degree C in the dark and analyze within 24 hours

Product References and Citations for Flow Cytometry Lysing Solution reagent

Bossuyt, X., Marti, G. E. & Fleisher, T. A. (1997) Cytometry 30, 124-33.
Fritsch, G., Printz, D., Stimpfl, M., Dworzak, M. N., Witt, V., Potschger, U. & Buchinger, P. (1997) Transfusion 37, 775-84.
Kormoczi, G. F., Wolfel, U. M., Rosenkranz, A. R., Horl, W. H., Oberbauer, R. & Zlabinger, G. J. (2001) J Immunol 167, 451-60.
Menendez, P., Redondo, O., Rodriguez, A., Lopez-Berges, M. C., Ercilla, G., Lopez, A., Duran, A., Almeida, J., Perez-Simon, J. A.,
San Miguel, J. F., Gratama, J. W. & Orfao, A. (1998) Cytometry 34, 264-71.

Other Notes

Small volumes of Flow Cytometry Lysing Solution reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Flow Cytometry Lysing Solution purchase

MBS570406 is a reagent targeting Flow Cytometry Lysing Solution. Also known as NM LYSE: Flow Cytometry Lysing Solution.
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