Rabbit Anti-SERPINA6 Polyclonal Antibody – Cat# MBS573049

Cortisol Binding Globulin (SERPINA6) Antibody – Rabbit Polyclonal

Name: SERPINA6 Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS573049
Price: 430 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS573049

Precipitating polyclonal rabbit antiserum to human cortisol binding globulin (transcortin) More details

$430 / 1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

cortisol binding globulin (SERPINA6), Polyclonal Antibody

Full Product Name

Rabbit anti Human cortisol binding globulin

Product Gene Name

anti-SERPINA6 antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiserum has not been tested in detail.

Form/Format

Delipidated, heat inactivated, lyophilized, stable whole serum.

Specificity

In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against human plasma a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. The antiserum does not react with any other protein component of human serum or plasma.

Preparation and Storage

Lyophilized at +4 degree C = at least 10 years
Reconstituted at or below -20 degree C = 3-5 years
Reconstituted at +4 degree C = 7 days

Handling:
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 degree C; prolonged storage at or below -20 degree C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum.
Diluted antiserum should be stored at +4 degree C, not refrozen, and preferably used the same day.

Molecular Weight

45,141 Da

Chromosome Location

Chromosome: 14; NC_000014.8 (94770585..94789688, complement). Location: 14q32.1

OMIM

122500

3D Structure

ModBase 3D Structure for P08185

Protein concentration

Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added.

Preservative

No preservative added.

Immunogen

Cortisol binding globulin is an alpha-1 glycoprotein with a molecular weight of 55,700. It is bonding and transporting cortisol in plasma. The mean concentration in adult serum is about 50 ug/ml. Elevated levels are observed in pregnancy. Freund's complete adjuvant is used in the first step of the immunization procedure.

Adsorption

Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Reactivity Note

Antibody titre

Precipitin titre not less than 1:16 when tested against normal human serum in agar-block immunodiffusion titration.

Intended use

In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of cortisol binding globulin or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques as ELISA and Western blotting if proper controls are included.

Directions for use

In immunoelectrophoresis use 2 III human plasma or equivalent against 120 III antiserum. In double radial immunodiffusion use a rosette arrangement with 10 III antiserum in 3 mm diameter center well and 2 III plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion use 1 percent antiserum in the gel.

Packing

Vial with 1 mllyophilized antiserum.

Caution

This antiserum should be handled by qualified persons only and appropriate precautions should be taken in its handling and disposal, and of all associated materials. For in vitro research purposes only.

Product Categories/Family for anti-SERPINA6 antibody

Antisera

Applications Tested/Suitable for anti-SERPINA6 antibody

Immunoprecipitation (IP)

Other Notes

Small volumes of anti-SERPINA6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SERPINA6 purchase

MBS573049 is an antibody targeting SERPINA6. Also known as Rabbit anti Human cortisol binding globulin. Reactive in Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiserum has not been tested in detail.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SERPINA6. It may not necessarily be applicable to this product.

NCBI GI #

73858564

NCBI GeneID

866

NCBI Accession #

NP_001747.2

UniProt Primary Accession #

P08185

UniProt Secondary Accession #

Q7Z2Q9; A8K456

UniProt Related Accession #

P08185

GenBank Nucleotide #

NM_001756.3

NCBI Official Full Name

corticosteroid-binding globulin

NCBI Official Synonym Full Names

serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6

NCBI Official Symbol

SERPINA6

NCBI Official Synonym Symbols

CBG

UniProt Protein Name

Corticosteroid-binding globulin

UniProt Synonym Protein Names

Serpin A6; Transcortin

Protein Family

Corticosteroid-binding globulin

UniProt Gene Name

SERPINA6

UniProt Synonym Gene Names

CBG; CBG

UniProt Entry Name

CBG_HUMAN

NCBI Protein Information

corticosteroid-binding globulin; serpin A6; transcortin; corticosteroid binding globulin; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6

NCBI Summary for SERPINA6

This gene encodes an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors which may have evolved by duplication events. [provided by RefSeq, Jul 2008]

UniProt Comments for SERPINA6

SERPINA6: Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species. Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency). CBG deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo/hypertension and muscle fatigue. Belongs to the serpin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q32.1

Cellular Component: extracellular space

Molecular Function: serine-type endopeptidase inhibitor activity; steroid binding

Biological Process: glucocorticoid metabolic process; transport

Disease: Corticosteroid-binding Globulin Deficiency

Research Articles on SERPINA6

1. Atomic interactions observed between progesterone and CBG explain the lower affinity of progesterone in comparison to corticosteroids.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SERPINA6 antibody

Reference Product	PubMed Publications
POMC antibody	>11 publications with SERPINA6 and POMC
NR3C1 antibody	>2 publications with SERPINA6 and NR3C1
SHBG antibody	>2 publications with SERPINA6 and SHBG
CRH antibody	>1 publications with SERPINA6 and CRH

Diseases associated with anti-SERPINA6 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>4 publications with SERPINA6 and Nervous System Diseases
Muscular Diseases Antibodies	>3 publications with SERPINA6 and Muscular Diseases
Neoplasms Antibodies	>2 publications with SERPINA6 and Neoplasms
Liver Diseases Antibodies	>2 publications with SERPINA6 and Liver Diseases
Inflammation Antibodies	>2 publications with SERPINA6 and Inflammation
Prenatal Exposure Delayed Effects Antibodies	>2 publications with SERPINA6 and Prenatal Exposure Delayed Effects
Hypertension Antibodies	>1 publications with SERPINA6 and Hypertension
Brain Diseases Antibodies	>1 publications with SERPINA6 and Brain Diseases
Liver Neoplasms Antibodies	>1 publications with SERPINA6 and Liver Neoplasms
Congenital Abnormalities Antibodies	>1 publications with SERPINA6 and Congenital Abnormalities

Organs/Tissues associated with anti-SERPINA6 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>10 publications with SERPINA6 and Liver
Ovary Antibodies	>2 publications with SERPINA6 and Ovary
Kidney Antibodies	>2 publications with SERPINA6 and Kidney
Heart Antibodies	>1 publications with SERPINA6 and Heart