Bacillus Cereus Phospholipase C Antibody – Rabbit Polyclonal

Polyclonal

Rabbit

Cross-reactivities against enzymes of other sources may occur but have not been determined.

Biotin-coupled hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). 1 vial with 1ml immunoconjugate, lyophilized from PBS

The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography

The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition.

The lyophilized conjugate is shipped at ambient temperature and may be stored at 4 degree C; prolonged storage at or below -20 degree C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below - 20 degree C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4 degree C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

A proprietary techniques for the binding to biotin is used, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assur stability and long shelf life.

No preservatived added, as it may interfere with the antibody activity. No foreign protein added

Phospholipase C isolated and purified from Bacillus cereus. Freund's complete adjuvant is used in the first step of the immunization procedure.

IgG protein concentration 10mg/ml. Biotin/IgG protein molar ration (B/P) approximately 5.4. No foreign proteins added.

N-Hydroxysuccinimidobiotin.

Bacterial; Fungal; Viral