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Fab Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS573193

The antiserum is reacting with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab’)2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double… More details

$430 / 1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Fab, Polyclonal Antibody
Full Product Name
Goat anti Rat Fab
Clonality
Polyclonal
Host
Goat
Species Reactivity
Rat
Form/Format
Delipidated, heat inactivated lyophilized stable whole serum No preservative added. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. No foreign proteins added
Specificity
Precipitating polyclonal Goat antiSerum to Rat Fab of IgG.
Preparation and Storage
The lyophilized antiserum may be stored at +4°C; prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Source
Purified Fab of normal rat IgG. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Reactivity Note
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in double radial immunodiffusion a reaction with Mouse and Human has been observed.

Product Categories/Family for anti-Fab antibody

Application Notes for anti-Fab antibody
Precipitation assays. In immunoelectrophoresis use 2 ul or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul antiserum in a 3 mm diameter centre well and 2 ul serum samples (neat and diluted) in 2 mm diameter peripheral wells.

Other Notes

Small volumes of anti-Fab antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Fab purchase

MBS573193 is an antibody targeting Fab. Also known as Goat anti Rat Fab. Reactive in Rat. Host: Goat.
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