EF-5 Inhibitor

Name: EF-5 Inhibitor; MyBioSource
Brand: MyBioSource
SKU: MBS5754819
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS5754819

MBS5754819 is an inhibitor targeting EF-5. EF-5 is an agent of hypoxia labeling used to identify hypoxia in cells. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

EF-5, Inhibitor

Full Product Name

EF-5

Product Gene Name

EF-5, inhibitor

Form/Format

Solid. Powder

Purity/Purification

98.00%

Preparation and Storage

Store at -20 degree C for 3 years powder
-80 degree C for 2 years in solvent

Molecular Weight

302.16

Solubility

DMSO: 125mg/mL (413.69mM) Need ultrasonic
(<1mg/ml refers to the product slightly soluble or insoluble)

Formula

C8H7F5N4O3

SMILES

O=C(NCC(F)(F)C(F)(F)F)CN1C=CN=C1[N+]([O-])=O

CAS Number

152721-37-4

Target & IC50

Others

In Vitro

EF-5 binding is a promising stratification biomarker for benzotriazine-N-oxide bioreductive prodrugs. Binding and detection using a monoclonal antibody in 9L gliomas are specific and oxygen-dependent, following intravenous injection of EF-5. Modification of tumor hypoxia causes similar changes to the bioreductive activation of both agents, resulting in a strong correlation between EF-5 binding and CEN209-induced DNA damage at the individual tumor level [1]. Detection of binding using fluorescence microscopy can be performed on frozen tissues; tissue sections can be counterstained with hematoxylin and eosin for light microscopic analysis. The distribution of hypoxia in a tumor can be inferred by examining individual tumor cells using flow cytometric techniques [2].

Product References and Citations for EF-5, inhibitor

Wang J, et al. The 2-nitroimidazole EF5 is a biomarker for oxidoreductases that activate the bioreductive prodrug CEN-209 under hypoxia. Clin Cancer Res. 2012 Mar 15;18(6):1684-95.
Wang J, et al. The 2-nitroimidazole EF5 is a biomarker for oxidoreductases that activate the bioreductive prodrug CEN-209 under hypoxia. Clin Cancer Res. 2012 Mar 15;18(6):1684-95.
Evans SM, et al. Identification of hypoxia in cells and tissues of epigastric 9L rat glioma using EF5 [2-(2-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl) acetamide]. Br J Cancer. 1995 Oct;72(4):875-82.
Evans SM, et al. Identification of hypoxia in cells and tissues of epigastric 9L rat glioma using EF5 [2-(2-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl) acetamide]. Br J Cancer. 1995 Oct;72(4):875-82.

Other Notes

Small volumes of EF-5, inhibitor vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

EF-5 Inhibitor

Product Overview

Structure of EF-5, inhibitor

Related Product Information for EF-5, inhibitor

Product References and Citations for EF-5, inhibitor

Other Notes