Bromobimane Inhibitor

Solid. Powder

98.00%

Store at -20 degree C for 3 years powder
-80 degree C for 2 years in solvent

271.11

DMSO: 86.6mg/mL (319.43mM)
(<1mg/ml refers to the product slightly soluble or insoluble)

C10H11BrN2O2

O=C1N(C(C(C)=C2C)=O)N2C(CBr)=C1C

71418-44-5

Others

Bromobimanes in solution (aqueous or organic solvents of medium polarity) react with small thiols, and with reactive protein thiol groups (e.g., hemoglobin). The reactions of bromobimanes with thiols are second-order and dependent on pH, the active nucleophile being the thiolate anion. The reaction of bromobimane with a thiolate converts the nonfluorescent agent into water-soluble fluorescent products [1]. The highly selective, rapid reactivity of bromobimanes toward thiols, the stability and fluorescence yield of the thiol derivatives, the ease of separation of the derivatives by reversed-phase HPLC, and the availability of both cell-penetrating and nonpenetrating forms, make the use bromobimanes an extremely powerful approach to the analysis of low molecular weight biothiols [2].