L-Leucine-p-nitroanilide Inhibitor

L-Leucine-p-nitroanilide is a substrate. It is used for the colorimetric determination of leucine aminopeptidase.

Lin SH, Van Wart HE. Effect of cryosolvents and subzero temperatures on the hydrolysis of L-leucine-p-nitroanilide by porcine kidney leucine aminopeptidase. Biochemistry.1982 Oct 26;21(22):5528-33. PubMed PMID: 7171573.
Shimasue A, Sugimoto Y, Tsubokura T. [No blank method using L-leucine-p-nitroanilide as substrate for the assay of serum leucine aminopeptidase (author's transl)]. Rinsho Byori. 1977 Feb;25(2):157-60. Japanese. PubMed PMID: 558393.
Zhu X, Barman A, Ozbil M, Zhang T, Li S, Prabhakar R. Mechanism of peptide hydrolysis by co-catalytic metal centers containing leucine aminopeptidase enzyme: a DFT approach. J Biol Inorg Chem. 2012 Feb;17(2):209-22. doi: 10.1007/s00775-011-0843-2. Epub 2011 Sep 15. PubMed PMID: 21918843.
Hernandez-Moreno AV, Villasenor F, Medina-Rivero E, Perez NO, Flores-Ortiz LF, Saab-Rincon G, Luna-Barcenas G. Kinetics and conformational stability studies of recombinant leucine aminopeptidase. Int J Biol Macromol. 2014 Mar;64:306-12. doi: 10.1016/j.ijbiomac.2013.12.023. Epub 2013 Dec 22. PubMed PMID: 24368112.

Small volumes of L-Leucine-p-nitroanilide, inhibitor vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS5756430 is an inhibitor targeting L-Leucine-p-nitroanilide.