A-674563 Inhibitor – 2hcl

Name: A-674563 Inhibitor; 2hcl; MyBioSource
Brand: MyBioSource
SKU: MBS5758263
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS5758263

A-674563 is an Akt1 inhibitor with Ki of 11nM, modest potent to PKA and >30-fold selective for Akt1 over PKC. More details

Please Inquire

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

A-674563 2HCl, Inhibitor

Full Product Name

A-674563 2HCl (552325-73-2(fb-2hcl))

Product Gene Name

A-674563, inhibitor

Form/Format

Solid. Powder

Purity/Purification

98.00%

Preparation and Storage

Store at -20 degree C for 3 years powder
-80 degree C for 2 years in solvent

Molecular Weight

431.36

Solubility

<1mg/ml refers to the product slightly soluble or insoluble

Formula

C22H24Cl2N4O

SMILES

Cl.Cl.Cc1n[nH]c2ccc(cc12)-c1cncc(OC[C@@H](N)Cc2ccccc2)c1

Cell Research

The cells on 96-well plates are gently washed with 200uL of PBS. Alamar Blue reagent is diluted 1:10 in normal growth media. The diluted Alamar Blue reagent (100 uL) is added to each well on the 96-well plates and incubated until the reaction is complete. The analysis is done using a fmax Fluorescence Microplate Reader, set at the excitation wavelength of 544nM and the emission wavelength of 595nM. Data are analyzed using SOFTmax PRO software. Cell lines:

Product Categories/Family for A-674563, inhibitor

PI3K/Akt/mTOR signaling; Cell Cycle/Checkpoint; GPCR/G Protein; Cytoskeletal Signaling; MAPK

Product References and Citations for A-674563, inhibitor

Luo Y, Shoemaker A R, Liu X, et al. Potent and selective inhibitors of Akt kinases slow the progress of tumors in vivo.[J]. Molecular Cancer Therapeutics, 2005, 4(6):977.
Zhu Q S, Ren W, Korchin B, et al. Soft Tissue Sarcoma Are Highly Sensitive to AKT Blockade: A Role for p53 Independent Up-regulation of GADD45alpha[J]. Cancer Research, 2008, 68(8):2895-2903.
Zou Y, Zhu Y R, Fan G, et al. Pre-clinical assessment of A-674563 as an anti-melanoma agent[J]. Biochemical & Biophysical Research Communications, 2016, 477(1):1-8.

Other Notes

Small volumes of A-674563, inhibitor vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for A-674563 purchase

MBS5758263 is an inhibitor targeting A-674563. Also known as A-674563 2HCl (552325-73-2(fb-2hcl)).