NSC95397 Inhibitor – NSC 95397

Name: NSC95397 Inhibitor; NSC 95397; MyBioSource
Brand: MyBioSource
SKU: MBS5758285
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS5758285

NSC 95397 is a potent inhibitor of Cdc25 dual specificity phosphatase(Ki of 32, 96, and 40nM for Cdc25A, Cdc25B, and Cdc25C, respectively). More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NSC 95397 (NSC95397), Inhibitor

Full Product Name

NSC 95397

Product Gene Name

NSC95397, inhibitor

Form/Format

Solid. Powder

Purity/Purification

97.12%

Preparation and Storage

Store at -20 degree C for 3 years powder
-80 degree C for 2 years in solvent

Molecular Weight

310.39

Solubility

DMSO: 100mg/mL (322.18mM)
(<1mg/ml refers to the product slightly soluble or insoluble)

Formula

C14H14O4S2

SMILES

O=C1C(SCCO)=C(SCCO)C(=O)c2ccccc21

CAS Number

93718-83-3

Target & IC50

Cdc25A: ic50 32nM (ki)
Cdc25B: ic50 96nM (ki)
Cdc25C: ic50 40nM (ki)

Cell Research

To measure the cell proliferation activity of NSC 95397 against colon cancer cells, SW480 (1 x 10^4), SW620 (2 x 10^4), and DLD-1 (1 x 10^4) cells were seeded into 96-well plates. After overnight culture, NSC 95397 cells were added at the indicated concentrations. After 24 hours of incubation, cell proliferation was determined in vitro using a BrdU cell proliferation assay kit. O.D. 450 values were analyzed by using a Multiskan PC. The apoptosis of colon cancer cells was determined by a PE Annexin V Apoptosis Detection Kit with 7-AAD. Briefly, cells were treated with the indicated concentration of NSC 95397 for 24 hours. After 24 h, Cells were harvested and stained with PE Annexin V/7-AAD for 15 minutes. The stained cells were analyzed using FACSCanto II low cytometer and FCS Express software[1]

In Vitro

NSC 95397 reduced cell viability and anchorage-independent growth of all the three colon cancer cell lines through inhibited proliferation and induced apoptosis via regulating cell-cycle-related proteins, including p21, cyclin-dependent kinases, and caspases[1].

Product Categories/Family for NSC95397, inhibitor

Phosphatase; Others

Product References and Citations for NSC95397, inhibitor

Navneet D, Bou-Yue P, Chien-Min L, et al. NSC 95397 Suppresses Proliferation and Induces Apoptosis in Colon Cancer Cells through MKP-1 and the ERK1/2 Pathway[J]. International Journal of Molecular Sciences, 2018, 19(6):1625-.
Navneet D, Bou-Yue P, Chien-Min L, et al. NSC 95397 Suppresses Proliferation and Induces Apoptosis in Colon Cancer Cells through MKP-1 and the ERK1/2 Pathway[J]. International Journal of Molecular Sciences, 2018, 19(6):1625-.
Park H, Carr B I, Li M, et al. Fluorinated NSC as a Cdc25 inhibitor[J]. Bioorganic and Medicinal Chemistry Letters, 2007, 17(8):2351-2354.
Park H, Carr B I, Li M, et al. Fluorinated NSC as a Cdc25 inhibitor[J]. Bioorganic and Medicinal Chemistry Letters, 2007, 17(8):2351-2354.
Dubey NK, et al. NSC 95397 Suppresses Proliferation and Induces Apoptosis in Colon Cancer Cells through MKP-1 and the ERK1/2 Pathway. Int J Mol Sci. 2018 May 31;19(6). pii: E1625.

Other Notes

Small volumes of NSC95397, inhibitor vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NSC95397 purchase

MBS5758285 is an inhibitor targeting NSC95397. Also known as NSC 95397.