Cytochalasin B Inhibitor

Name: Cytochalasin B Inhibitor; MyBioSource
Brand: MyBioSource
SKU: MBS5758286
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS5758286

Cytochalasin B is a mycotoxin binding to the barbed end of actin filaments. It can disrupt the formation of actin polymers (Kd: 1.4-2.2nM for F-actin). More details

Please Inquire

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Cytochalasin B, Inhibitor

Full Product Name

Cytochalasin B

Product Synonym Names

Phomin

Form/Format

Solid. Powder

Purity/Purification

98.00%

Preparation and Storage

Store at -20 degree C for 3 years powder
-80 degree C for 2 years in solvent

Molecular Weight

479.61

Solubility

DMSO: 20mg/mL (41.7mM)
Ethanol: 20mg/mL (41.7mM)
(<1mg/ml refers to the product slightly soluble or insoluble)

Formula

C29H37NO5

SMILES

C[C@@H]([C@@H]([C@H](Cc1ccccc1)NC1=O)[C@@]1([C@H]([C@@H]1O)/C=C/C[C@H](C)CCC[C@H](/C=C2)O)OC2=O)C1=C

CAS Number

14930-96-2

Cell Research

The attached cell lines M109c, B16BL6, and B16F10 are seeded at 1 to 4x10^4 cells/mL in 2mL volumes in 24-well culture plates 1 day prior to treatment with Cytochalasin B. The suspension culture of P388/ADR cells is seeded at 5x10^4 cells/mL and allowed to grow overnight before Cytochalasin B treatment, Cells are treated with Cytochalasin B for 3 h, as well as 2, 3, or 4 days. In the case of continuous exposure for 2, 3, or 4 days, attached cells are trypsinized and counted with a hemacytometer. Leukemia cell suspensions are counted with a Coulter Counter. In the case of short-term exposure, cells are washed twice with fresh medium, then trypsinized (except for P388/ADR cells), reseeded, and allowed to regrow for 3 days, at which time they are counted. Growth results are calculated as the number of cells generated above the seeding density compared to the untreated control cells and graphically presented as a percent of control increase [3].

In Vitro

Cytochalasin B (10, 25, 50mg/kg, i.p.) dose-dependently increases the life expectancy of Balb/c mice bearing with P388/ADR leukemias. Cytochalasin B at 50mg/kg produces 10% long-term survival in the multidrug-resistant P388/ADR cohort, and 40% long-term survival in the drug-sensitive P388/S cohort [3].

Product Categories/Family for Cytochalasin B, inhibitor

Cytoskeletal Signaling

Product References and Citations for Cytochalasin B, inhibitor

Theodoropoulos PA, et al. Cytochalasin B may shorten actin filaments by a mechanism independent of barbed end capping. Biochem Pharmacol. 1994 May 18;47(10):1875-81.
Theodoropoulos PA, et al. Cytochalasin B may shorten actin filaments by a mechanism independent of barbed end capping. Biochem Pharmacol. 1994 May 18;47(10):1875-81.
Zhou C, et al. The effect of Cytochalasin B and Jasplakinolide on depolymerization of actin filaments in goose muscles during postmortem conditioning. Food Res Int. 2016 Dec;90:1-7.
Zhou C, et al. The effect of Cytochalasin B and Jasplakinolide on depolymerization of actin filaments in goose muscles during postmortem conditioning. Food Res Int. 2016 Dec;90:1-7.
Trendowski M, et al. Chemotherapy with cytochalasin congeners in vitro and in vivo against murine models. Invest New Drugs. 2015 Apr;33(2):290-9.
Trendowski M, et al. Chemotherapy with cytochalasin congeners in vitro and in vivo against murine models. Invest New Drugs. 2015 Apr;33(2):290-9.

Other Notes

Small volumes of Cytochalasin B, inhibitor vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Cytochalasin B purchase

MBS5758286 is an inhibitor targeting Cytochalasin B.