AM-0902 Inhibitor

Solid. Powder

100.00%

Store at -20 degree C for 3 years powder
-80 degree C for 2 years in solvent

370.79

DMSO: 145mg/mL (391.05mM) Need ultrasonic and warming
(<1mg/ml refers to the product slightly soluble or insoluble)

C17H15ClN6O2

Cn(cn1)c2c1N=CN(Cc1nc(CCc(cc3)ccc3Cl)no1)C2=O

1883711-97-4

On the day of assay, culture media is removed and MDCK cells are incubated for 10 minutes at room temperature (RT) with 50uL of AM-0902 in AM-0902 dilution buffer [HBSS containing 1mM HEPES+0.1mg/mL BSA] at final concentrations (2.0nM to 40uM, 1:3 ratio), followed by another 3 min incubation at RT. The reaction mixture is aspirated, and cells are washed three times with PBS containing 0.1mg/mL BSA. Radioactivity is measured using a TopCount microplate scintillation counter. The activation of TRPA1 is measured by the cellular uptake of radioactive calcium.

Membrane transporter/Ion channel; TRP/TRPV Channel